黑老虎果实提取物对小鼠腹泻模型的干预作用

Effects of extracts from fruit of kadsura on diarrhea model in mice

关键词:  黑老虎果实提取物  小鼠腹泻模型  干预  

Key words:  fruit of kadsura  Mouse diarrhea model  Intervention

摘要:

为了研究黑老虎果实提取物的抗腹泻作用，从而为临床应用提供实验依据。实验首先对黑老虎果实提取物中木脂素、三萜及黄酮含量进行测定，然后对ICR小鼠进行细菌性腹泻、药物性腹泻和小肠炭末推进实验。根据实验要求，将细菌性腹泻模型小鼠和药物性腹泻模型小鼠灌胃不同药物，连续7天，除正常组外，其余小鼠于末次给药30min后分别腹腔注射大肠埃希菌溶液和灌胃番泻叶提取液，观察并记录各组小鼠腹泻情况；小肠炭末推进实验于小鼠末次给药30min后灌胃给予5%炭末，计算小肠炭末推进率 ；结果表明，黑老虎果实提取物中木脂素类、三萜类及黄酮类化合物的含量分别为44.48%、4.57%、14.02%黑老虎果实提取物各剂量组对细菌性小鼠均有较好的预防效果，其中高剂量组 （200mg/kg）最佳，与模型组呈显著差异（P＜0.01），效果亦优于阳性药物组；提取物各剂量组均能减少番泻叶腹泻小鼠稀便数量，且与模型组呈显著差异，但对潜伏时间没影响；提取物对小鼠小肠炭末推进无明显影响 由此可知，黑老虎果实提取物对细菌性腹泻小鼠具有良好的抗腹泻作用，对药物性致腹泻作用较弱。

Abstract:

Abstract:To study the anti-diarrhea effect of the fruit of kadsura extract, providing experimental basis for clinical application.The experiment first determined the content of lignans, triterpenes and flavonoids in kadsura fruit extracts，ICR mice were subjected to bacterial diarrhea, drug-induced diarrhea, and small intestinal charcoal propulsion experiments .According to the experimental requirements, the bacterial diarrhea model mice and the drug-induced diarrhea model mice were intragastrically administered with different drugs for 7 consecutive days. Except for the normal group, the remaining mice were intraperitoneally injected with Escherichia coli solution and 30 minutes after the last administration. Gavage senna leaf extract, observe and record the diarrhea of each group of mice; small intestine charcoal advancing experiment 30 minutes after the last administration of mice, 5% charcoal was given by gavage to calculate the intestinal charcoal advancing rate. The results showed that the contents of lignans, triterpenoids and flavonoids were 44.48%, 4.57% and 14.02%.Each dose group of kadsura fruit extract has a good preventive effect on bacterial mice, among which the high dose group (200 mg/kg) is the best, which is significantly different from the model group (P<0.01) , The effect is also better than the positive drug group; each dose group of the extract can reduce the number of loose stools in mice with senna diarrhea, and is significantly different from the model group, but has no effect on the incubation time; the extract has no effect on the intestinal charcoal of mice No obvious impact on advancement.From this we can see:The fruit of kadsura extract has good anti-diarrhea effect on mice with bacterial diarrhea, but weaker effect on drug-induced diarrhea.