刺五加多糖对雏鸡脾脏中CD4+ 和CD8+T淋巴细胞定位分布的影响

Effects of Acanthopanax senticosus Polysaccharide on distribution of CD4+ and CD8+ T lymphocytes in chicken spleen

关键词:  刺五加多糖  脾脏  CD4+ T淋巴细胞  CD8+ T淋巴细胞  鸡  定位分布

Key words:  Acanthopanax senticosus polysaccharide  spleen  CD4+ T lymphocytes  CD8+ T lymphocytes  chicken  distribution

摘要:

为了研究刺五加多糖（ASPS）对雏鸡脾脏中CD4+和CD8+ T淋巴细胞定位分布的影响，从组织学角度评价ASPS对脾脏的免疫调节作用，试验将1日龄海兰褐公雏饲养至7日龄时选取150只，随机分为3组：空白对照组、ASPS低剂量组（ASPSL）和高剂量组（ASPSH），每组50只，所有组每天注射1次，连续注射3天。免疫后的第7、14、21和28天分别取其脾脏制作冰冻切片，采用免疫组织化学方法检测CD4+和CD8+ T淋巴细胞的定位分布。结果显示，与空白对照组比较，免疫注射后21天和28天时ASPSL组和ASPSH组CD4+ T淋巴细胞的数量均显著增加（P<0.05），而且ASPS能够促进红髓中CD4+ T淋巴细胞向动脉周围淋巴鞘迁移，从而使单个动脉周围淋巴鞘面积较对照组明显增加，而ASPS对脾脏中CD8+ T淋巴细胞的数量和分布无明显影响。由此可知，ASPS能够通过影响脾脏中CD4+ T淋巴细胞的定位分布发挥免疫调节作用，这对于进一步揭示ASPS的免疫调节机制具有重要意义。

Abstract:

In order to investigate the effect of Acanthopanax senticosus polysaccharide (ASPS) on the localization and distribution of CD4+ and CD8+ T lymphocytes in the spleen of chicks, and evaluate the immunomodulatory effect of ASPS on spleen from the perspective of histology, 150 1-day-old Hy-Line brown male chicks were randomly divided into 3 groups with 50 chicks in each group: blank control group, ASPS low-dose group (ASPSL) and ASPS high-dose group (ASPSH). All groups were injected once a day for 3 consecutive days. The distribution of CD4+ and CD8+ T lymphocytes was detected by immunohistochemistry at 7, 14, 21 and 28 days after immunization. The results showed that compared with blank control group, the number of CD4+ T lymphocytes in ASPSL and ASPSH groups was significantly increased at 21 and 28 days after immunization (P<0.05), and ASPS could promote the migration of CD4+ T lymphocytes from the red pulp to the periarterial lymphatic sheath, so that the area of single periarterial lymphatic sheath was significantly increased compared with the control group. However, ASPS had no significant effect on the number and distribution of CD8+ T lymphocytes in spleen. The results revealed that ASPS could play an immunomodulatory role by affecting the localization and distribution of CD4+ T lymphocytes in the spleen, which is of great significance for further revealing the immunomodulatory mechanism of ASPS.