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HPLC法测定芪蓝囊病饮中的靛玉红 |
牛华星,章安源,赵见,郭腾,李有志,尹伶灵,刘少宁,陈玲,魏秀丽,张志民,张传津 |
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(山东省饲料兽药质量检验中心;山东省兽药质量检验所) |
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摘要: |
建立了芪蓝囊病饮中靛玉红的高效液相色谱(HPLC-PDA)检查方法。采用C18(250 mm×4.6 mm×5 μm),柱温35 ℃,以乙腈-0.2%磷酸水进行梯度洗脱,波长采集范围为200~640 nm,流速为1.0 mL/min,进样量10 L,提取检测波长为289 nm。靛玉红在0.5~2.0 μg(R2 =0.9991)线性关系良好,平均加样回收率90.0%(RSD=1.36%,n=6)。所建方法色谱分离较好,前处理简单,可用于芪蓝囊病饮中的靛玉红的含量测定,为完善芪蓝囊病饮的质量标准提供依据。 |
关键词: 靛玉红 芪蓝囊病饮 高效液相色谱法 |
DOI: |
投稿时间:2021-01-17修订日期:2021-06-11 |
基金项目: |
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Determination of Indirubin in Qilan Nang Disease Decoction by HPLC |
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(Shandong Center for Quality Control of Feed and Veteriary Drugs) |
Abstract: |
A HPLC-PDA method for the determination of indirubin in Qilan Nange disease decoction was established.The C18 (250 mm×4.6 mm×5 μm) column temperature was 35 ℃, and the gradient elution was performed with acetonitrile-0.2% phosphoric acid water. The wavelength collection range was 200~640 nm, the flow rate was 1.0 mL/min, the injection volume was 10 μL, and the extraction detection wavelength was 289 nm.The linear range of indirubin was 0.5~2.0 μg (R2=0.9991). The average recoveries were 90.0% (RSD=1.36%,n=6).The established method has good chromatographic separation and simple pretreatment, which can be used for the determination of indirubin in Qilan Nang disease decoction, and provides a basis for improving the quality standard of Qilan Nang disease decoction. |
Key words: indirubin Qilan Nang disease decoction HPLC |