参金止痢口服液对腹泻哺乳仔猪肠道微生物菌群影响的差异性分析

Effect of Shenjin Zhili Oral Liquid on Intestinal Microflora of Suckling Piglets with Diarrhea

关键词:  参金止痢口服液  腹泻  哺乳仔猪  肠道菌群

Key words:  Shenjin Zhili Oral Liquid  diarrhea  suckling piglets  intestinal flora

摘要:

研究参金止痢口服液对腹泻哺乳仔猪肠道微生物菌群的影响。选取同等饲养条件下7~15日龄体重相近的“杜×长×大”三元杂交仔猪共计9头,其中有腹泻症状的6头将其随机平均分为腹泻空白组(FXK)和腹泻中药组(FZ),正常的3头为健康空白组(JK),中药组灌服参金止痢口服液,空白组灌服同等体积生理盐水,连续灌胃5d,在给药后的第8天对仔猪采血进行抗体及细胞因子水平测定,并采集结肠内容物提取样本中菌群总DNA进行16S rRNA高通量测序。结果显示：与FXK组相比,FZ组血清中IgG含量显著升高(P＜0.05),IgM含量显著降低(P＜0.05),炎性因子IL-1β、IL-6、TNF-α含量显著下调(P＜0.01)。α、β多样性分析结果表明,三个组结肠肠道菌群结构差异显著。在门分类水平上,FZ组仔猪肠道拟杆菌门相对丰度显著高于FXK组(P＜0.05),变形菌门相对丰度显著低于FXK组(P＜0.05),而FZ与JK组之间两个菌门差异不显著。属水平上,FZ组韦荣球菌属和乳杆菌属相对丰度显著低于FXK组(P＜0.05),普雷沃氏菌属相对丰度显著高于FXK组(P＜0.05),FZ组与JK组之间这三个菌属差异不显著。综上结果表明,参金止痢口服液可能通过调节免疫及炎性相关因子水平和调节肠道菌群结构从而发挥治疗哺乳仔猪腹泻的作用。

Abstract:

AIM To study the effect of Shenjin Zhili Oral Liquid on the intestinal microflora of suckling piglets. METHODS 9 “Du×Long×Large” three-way crossbred piglets with similar weights from 7 to 15 days old under the same feeding conditions were selected, among which 6 pigs with diarrhea symptoms were randomly divided into diarrhea blank group (FXK) and diarrhea Chinese medicine Group (FZ), the healthy 3 are the healthy blank group (JK), the traditional Chinese medicine group is given Shenjin Zhili Oral Liquid, and the blank group is given the same volume of normal saline for 5 days continuously, on the 8th day the Blood was collected from each piglets to determine antibody and cytokine levels, and colon contents were collected to extract the total DNA of the bacterial population of each sample for 16S rRNA high-throughput sequencing. RESULTS The results showed that compared with the FXK group, the serum IgG content in the FZ group was significantly increased(P＜0.05), the IgM content was significantly reduced(P＜0.05), and the inflammatory factors IL-1β, IL-6, and TNF-α content were significant reduced(P＜0.01). The results of α and β diversity analysis showed that the structure of colonic intestinal flora in the three groups was significantly different. At the phyla level, the relative abundance of Bacteroides in the intestinal tract of piglets in the FZ group was significantly higher than that in the FXK group(P＜0.05), and the relative abundance of the Proteobacteria was significantly lower than that in the FXK group(P＜0.05), while the difference of phyla between FZ and JK were not significant. At the genus level, the relative abundance of Veillonella and Lactobacillus in the FZ group was significantly lower than that in the FXK group(P＜0.05), and the relative abundance of Prevotella was significantly higher than that in the FXK group(P＜0.05), while the difference of genus between FZ and JK were not significant. CONCLUSION These results indicate that Shenjin Zhili Oral Liquid may play a role in treating diarrhea in suckling piglets by regulating the level of immune and inflammatory related factors and regulating the structure of intestinal flora.