兽药典收载羊败血性链球菌病疫苗生产检验用菌种的系统分类鉴定

Systematic identification of CVCC 553，55001 and 55002 for producing and inspecting vaccine against Ovine/Caprine Septicemia Streptococcosis in Chinese Veterinary Pharmacopoeia

关键词:  羊败血性链球菌病  马链球菌反刍亚种  Biolog  16S rRNA  全基因组序列分析

Key words:  ovine/caprine Streptococcosis septicemia  Streptococcus equi subsp. ruminatorum  Biolog  16S rRNA  whole genome sequence analysis

摘要:

根据现有细菌分类鉴定方法，采用血清学、Biolog、16S rRNA序列分析和全基因组序列分析等方法对羊败血性链球菌病疫苗生产检验用菌种CVCC 553、55001和55002进行系统鉴定。结果显示，菌种CVCC 553、55001和55002血清群为兰氏C群；Biolog鉴定为马链球菌反刍亚种；16S rRNA序列分析与马链球菌反刍亚种模式菌株CECT 5772的同源性最高（均为99.72%），且在系统发育树中位于同一分支；与马链球菌反刍亚种模式菌株CCUG 47520的DNA-DNA的杂交值大于70%，分别为80.5%、80.2%、80.5%，与马链球菌反刍亚种模式菌株CECT 5772的平均核苷酸同源性分别为97.73%、97.64%、97.77%。因此本研究认为羊败血性链球菌病疫苗生产用菌种CVCC 553、55001和55002不是原命名的马链球菌兽疫亚种，应为马链球菌反刍亚种。

Abstract:

According to the existing methods of bacterial classification, the strains CVCC 553, 55001 and 55002, which used to producing and inspecting vaccine against ovine/caprine streptococcusis septicemia, were systematically identified by serology, BIOLOG, 16S rRNA sequence analysis and whole genome sequence analysis. The results showed that CVCC 553, 55001 and 55002 were Lancefield group C, identified as Streptococcus equi subsp ruminatorum by Biolog. Comparative 16S rRNA gene sequencing studies confirmed that CVCC 553, 55001 and 5502 are members of the species Streptococcus equi, with Streptococcus equi subsp. ruminatorum CECT 5772 as their closest phylogenetic relative (99.72%). DNA-DNA pairing studies showed that CVCC 553, 55001 and 55002 displayed more than 70% relatedness to Streptococcus equi subsp. ruminatorum type strain CCUG 47520 (80.5%、80.2%、80.5%). Average nucleotide identity between CVCC 553, 55001, 55002 and Streptococcus equi subsp. ruminatorum type strain CECT 5772 is 97.73%、97.64%、97.77%. In summary, CVCC 553, 55001 and 55002 are not Streptococcus equi subsp. zooepidemicus, but Streptococcus equi subsp. ruminatorum.