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HPLC法测定天然植物粗提物中芍药苷含量
杜西翠,李云波,张趁,郭艳钦,徐丹,刘格格,王梦雪
0
(河北锦坤动物药业有限公司;河北省兽用中药制剂技术创新中心;河北省饲用天然植物提取物产业技术创新战略联盟;辛集市中兽药技术创新中心;石家庄市汇丰动物保健品有限公司;河北省农业创新驿站)
摘要:
建立高效液相色谱法检测天然植物粗提物饲料原料中芍药苷的测定方法。用十八烷基硅烷键合硅胶色谱柱,以乙腈-0.1%磷酸溶液(14:86)为流动相,检测波长230nm,流速:1.0 mL/min;柱温:30 ℃;进样量:10μL。结果显示,芍药苷的线性方程为Y=10.6017X-14.8514,(r=0.99966,n=6),本方法的回收率为100.4~102.1%,RSD均小于2.0%。结果表明,本方法准确,可靠,适用于天然植物粗提物饲料原料中芍药苷的测定。
关键词:  HPLC  天然植物粗提物  饲料原料  芍药苷
DOI:
投稿时间:2020-09-02修订日期:2021-03-10
基金项目:河北省人才工程培养资助项目(A201901135) ,4,李云波2,张趁6,郭艳钦1,徐丹3,刘格格1,王梦雪5
Determination of paeoniflorin in natural plant crude extract feed materials by HPLC
(Hebei Jinkun Animal Pharmaceutical Co LTD;China;Hebei Veterinary Medicine Preparation Technology Innovation Center;China;Industrial technology Innovation strategic Alliance of Forage natural plant extracts in Hebei Province;China;Xinji Veterinary medicine Technology Innovation Center;China;Shijiazhuang Huifeng Animal Health Products Co LTD;China;Hebei Province Agricultural Innovation Post;China)
Abstract:
To establish a HPLC method for the determination of paeoniflorin in the crude extracts of natural plants. The mobile phase was acetonitrile-0.1% phosphoric acid solution (14:86), the detection wavelength was 230nm, the flow rate was 1.0 ml / min, the column temperature was 30 ℃, and the injection volume was 10 μ L. The results showed that the linear equation of paeoniflorin was Y = 10.6017X-14.8514, (r = 0.99966, n = 6). The recoveries of paeoniflorin were 100.4 ~ 102.1%, RSD were less than 2.0%. The results showed that the method was accurate and reliable, and could be used for the determination of paeoniflorin in feed materials of natural plant crude extract.
Key words:  HPLC  crude extract of natural plants  feed material  paeoniflorin

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