解淀粉芽胞杆菌固态发酵当归培养基的研究

Study on Angelica medium for solid fermenting Radix?by Bacillus amyloliquefaciens

关键词:  当归  解淀粉芽胞杆菌  固体发酵培养基

Key words:  Angelica  Bacillus amyloliquefaciens  solid fermenting Radix

摘要:

为筛选和优化解淀粉芽胞杆菌固态发酵当归培养基，以当归多糖含量为指标，采用单因素试验对当归固态发酵培养基成分筛选，采用响应面分析试验对培养基组分进行优化。结果显示，在所筛选成分中，最适宜菌株发酵的固态培养基成分为当归、黄豆粉、碳酸钙和水。优化的固态发酵培养基各组分含量分别为：当归320.914 g/L、 黄豆粉109.918 g/L、碳酸钙1.532 g/L、水544.159 mL/L，37℃发酵培养72 h，固态发酵物中的多糖质量分数达24.91 mg/g。结果表明，优化的当归固态发酵培养基适宜解淀粉芽胞杆菌生长，易于多糖的释放，所筛选的发酵培养基原材料符合固态发酵产业化生产条件要求。

Abstract:

In order to screen and optimize medium for solid fermenting Angelica by Bacillus amyloliquefaciens，The components and compositions of angelica solid fermentation medium were screened and optimized by the single factor experiments，response surface methodology utilising the growth amount of bacteria and the polysaccharide as an indicators. In results，the optimum components of medium were angelica，soybean flour and calcium carbonate.The optimum medium was consisted of 320.914 g/L angelica，109.918 g/L soybean flour，1.532 g/L calcium carbonate and 544.159 mL/L water. The content of polysaccharide of B. amyloliquefaciens cultured in the optimised medium for 72 h at 37℃ was 24.91 mg/g. In conclusion，the optimized solid fermentation medium was suitable for the growth of B. amyloliquefaciens ，polysaccharide release easily, materials conform to the requirements of industrial production conditions of solid fermentation.