HPLC法同时测定板青败毒口服液中(R-S)告依春、绿原酸、咖啡酸的含量

Determination of chlorogenic acid 、(Ｒ-S)–goitrin、 and caffeic cid in Qibanqing Granule by HPLC.LI You-zhi1 ,ZHANG An-yuan1, ZHANG Chuan-jin1, CHEN-Ling1, WEI XiU-Li1,

关键词:  ［］ 高效液相色谱法  板青败毒口服液  (R-S)告依春  绿原酸  咖啡酸

Key words:  HPLC  Banqingbaidu oral liquid  (Ｒ-S) goitrin  Chlorogenic acid  Caffeic acid  

摘要:

［］ 建立板青败毒口服液中(R-S)告依春、绿原酸、咖啡酸含量测定方法。使用C18(250mm× 4.6 mm,5μm,)色谱柱,样品前处理用50%乙醇稀释,乙腈︰0.05%磷酸(8︰92,V/V)梯度洗脱,采用光二极管阵列检测器(PAD),波长采集范围为 200～400 nm,流速为 0.8 mL/min,进样量为10μL,柱温30℃,提取波长是245 nm。结果表明,绿原酸、咖啡酸在5.0～400μg(R²= 0.9998、R²= 0.9993)呈良好的线性关系,(R-S)告依春在2.5～300μg(R²= 0.9991)呈良好的线性关系；平均加样回收率分别为91.1%、92.9%、91.3% (n= 6)；RSD分别为1.0%、1.5% 、1.4%。本方法简单、准确、重复性好,适用于板青败毒口服液的质量控制。

Abstract:

A detection method of(Ｒ-S)–goitrin,chlorogenic acid and caffeic acid in Banqingbaidu oral liquid.It was established by HPLC. Samples directly diluted by 50% ethanol solution。The mobile phase was acetonitrile ：0.05% phosphoric acid solution (8∶92,V/V), High performance liquid chromatography with photo-diode array detector.Photo-diode array detector wavelength range was 200 ～ 400 nm,the flow rate was 0.8 mL /min,and the injection volume was 10 μL,the column temperature was 30℃,The recorded wave length was 245 nm. The test results show that chlorogenic acid and caffeic acid had a good linear relationship in the range of 5.0～400 μg/mL(Ychlorogenic acid = 0.0028X－0.2311,R²= 0.9998；Ycaffeic acid = 0.0167X+0.0014,R²= 0.9993)respectively.(Ｒ-S)–goitrin was in linear over the range of 2.5 to 300μg/mL(Y(Ｒ-S)goitrin = 0.0122X－0.1096,R² = 0.9991).The average recovery rate (n=6) was 91.1%, 92.9% and 91.3% respectively, and the RSD was 1.0%,1.5% and 1.4% respectively. The method was accurate and simple, which can be provided a reliable way for the quality control Banqingbaidu oral liquid.