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HPLC法同时测定板青败毒口服液中(R-S)告依春、绿原酸、咖啡酸的含量
章安源,李有志,张传津,陈玲,魏秀丽,薄永恒,杨修镇,惠庆亮,张淑二,田学磊,罗国栋,牛华星
0
(山东省兽药质量检验所)
摘要:
[] 建立板青败毒口服液中(R-S)告依春、绿原酸、咖啡酸含量测定方法。使用C18(250mm× 4.6 mm,5μm,)色谱柱,样品前处理用50%乙醇稀释,乙腈︰0.05%磷酸(8︰92,V/V)梯度洗脱,采用光二极管阵列检测器(PAD),波长采集范围为 200~400 nm,流速为 0.8 mL/min,进样量为10μL,柱温30℃,提取波长是245 nm。结果表明,绿原酸、咖啡酸在5.0~400μg(R2= 0.9998、R2= 0.9993)呈良好的线性关系,(R-S)告依春在2.5~300μg(R2= 0.9991)呈良好的线性关系;平均加样回收率分别为91.1%、92.9%、91.3% (n= 6);RSD分别为1.0%、1.5% 、1.4%。本方法简单、准确、重复性好,适用于板青败毒口服液的质量控制。
关键词:  [] 高效液相色谱法  板青败毒口服液  (R-S)告依春  绿原酸  咖啡酸
DOI:
投稿时间:2020-06-30修订日期:2020-08-25
基金项目:
Determination of chlorogenic acid 、(R-S)–goitrin、 and caffeic cid in Qibanqing Granule by HPLC.LI You-zhi1 ,ZHANG An-yuan1, ZHANG Chuan-jin1, CHEN-Ling1, WEI XiU-Li1,
((1. Institute of Veterinary Drug Quality Inspection of Shandong Provincial,)
Abstract:
A detection method of(R-S)–goitrin,chlorogenic acid and caffeic acid in Banqingbaidu oral liquid.It was established by HPLC. Samples directly diluted by 50% ethanol solution。The mobile phase was acetonitrile :0.05% phosphoric acid solution (8∶92,V/V), High performance liquid chromatography with photo-diode array detector.Photo-diode array detector wavelength range was 200 ~ 400 nm,the flow rate was 0.8 mL /min,and the injection volume was 10 μL,the column temperature was 30℃,The recorded wave length was 245 nm. The test results show that chlorogenic acid and caffeic acid had a good linear relationship in the range of 5.0~400 μg/mL(Ychlorogenic acid = 0.0028X-0.2311,R2= 0.9998;Ycaffeic acid = 0.0167X+0.0014,R2= 0.9993)respectively.(R-S)–goitrin was in linear over the range of 2.5 to 300μg/mL(Y(R-S)goitrin = 0.0122X-0.1096,R2 = 0.9991).The average recovery rate (n=6) was 91.1%, 92.9% and 91.3% respectively, and the RSD was 1.0%,1.5% and 1.4% respectively. The method was accurate and simple, which can be provided a reliable way for the quality control Banqingbaidu oral liquid.
Key words:  HPLC  Banqingbaidu oral liquid  (R-S) goitrin  Chlorogenic acid  Caffeic acid  

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