固体脂质纳米粒理化性质对纳米粒吸附蛋白的影响

The effect of physicochemical properties of solid lipid nanoparticles on its surface adsorption of protein

关键词:  固体脂质纳米粒  理化性状  表面吸附  蛋白载体

Key words:  Solid lipid nanoparticles  Physicochemical properties  Surface adsorption  Protein carrier

摘要:

蛋白载体在兽医药有特定的研究价值和应用前景,我们前期研究证实固体脂质纳米粒(Solid lipid particles, SLN)表面对不同蛋白有较强的吸附作用,是良好蛋白的载体。本文通过改变脂质材料、乳化时间或加入表面修饰材料等制备了不同性状的SLN,并以BSA为标志蛋白进一步研究了纳米粒理化性质对吸附的影响。结果显示,纳米粒主要理化性质对吸附率有显著影响：不同脂质材料制备的纳米粒对蛋白的吸附率不同；小粒径纳米粒吸附能力更强；正电位纳米粒吸附率显著高于负电位纳米粒；但乳化剂浓度对吸附率无明显影响。这些结果为固体脂质纳米粒作为蛋白载体的制备及组方优化提供依据,为提高载蛋白效率提供帮助。

Abstract:

The protein carrier has specific research value and application prospects for veterinary medicine. Our previous research confirmed that solid lipid nanoparticles (SLN) are promising protein carriers through surface adsorption. In this paper, SLNs with different properties were prepared by changing lipid materials, emulsification time, or adding surface modification materials. Using BSA as a marker protein, the effects of nanoparticle physicochemical properties on adsorption were further studied. The results showed that the main physicochemical properties of nanoparticles had significant effects on the adsorption rates. The adsorption rates of proteins on the nanoparticles prepared by different lipid materials were different. The adsorption capacity of smaller size nanoparticles was stronger than that of the larger ones. The adsorption rate of proteins on the positive potential nanoparticles was significantly higher than that of negative potential nanoparticles. But the emulsifier concentration has no significant effect on the adsorption rate. These results provide a basis for the preparation and optimization of solid lipid nanoparticles as protein carriers, and could help to improve the efficiency of protein loading.