猪链球菌2型的分离鉴定及致病性试验

Isolation,Identification and Pathogenicity of Streptococcus suis Type 2

关键词:  猪链球菌  分离  鉴定  致病性

Key words:  Streptococcus suis  isolation  identification  pathogenicity

摘要:

从全国部分猪场采集疑似猪链球菌感染病例脑样品20份进行细菌分离，成功分离到10株细菌，通过猪链球菌及血清类型鉴定，最终确定其中一株为2型猪链球菌。应用猪链球菌7种主要毒力因子特异性基因扩增检测方法检测所分离到的2型猪链球菌的毒力因子分布情况，并应用小鼠攻毒试验对其致病性进行观察研究。结果表明：分离的2型猪链球菌具备7种毒力因子；动物试验表明SS2能引起小鼠的急性败血症及脑膜炎；细菌回归试验结果表明，试验组死亡小鼠的脑、心脏、肝脏、脾脏、肺脏、肾脏均有细菌定植，且能分离出攻毒菌。此分离菌株的研究为研制2型猪链球菌病疫苗奠定了基础。

Abstract:

Twenty brain samples of pig with suspected infection by Streptococcus suis were collected from some pig farms in the country for bacterial isolation, and ten strains of bacteria were successfully isolated. One strain was Streptococcus suis type 2 through the identification. Seven major virulence factors specific gene amplification assays of Streptococcus suis were used to detect the distribution of the virulence factors in the isolated Streptococcus suis type 2, and the pathogenicity test was conducted to observe the pathogenicity of the mice. The results showed that the isolation of Streptococcus suis type 2 possessed 7 virulence factors; animal tests showed that Streptococcus suis type 2 could cause acute septicemia and meningitis in mice.The bacteria in brain, heart, liver, spleen, lungs and kidneys of the experimental group were all colonized and the challenge bacteria could be isolated. The study of the isolated strains laid the foundation for developing the vaccine of Streptococcus suis type 2.