六种传代细胞系的致瘤性研究

Tumorigenicity of Six Cell Lines

关键词:  传代细胞系  裸鼠  致瘤性

Key words:  cell line  nude mice  tumorigenicity

摘要:

为分析6种传代细胞系对裸鼠致瘤性的影响，将80只裸鼠随机分为8组，每组10只。处理组分为MDBK细胞组、OA3.Ts细胞组、Marc-145细胞组、McCoy细胞组、PK-15细胞组、ST细胞组，每种细胞培养物颈背部皮下注射10只裸鼠。阳性对照组BHK-21细胞培养物和阴性对照组鸡胚成纤维细胞培养物各注射10只裸鼠。在饲养5周和12周后对裸鼠剖检并进行病理组织学检测。结果发现，阳性对照BHK-21细胞组、McCoy细胞组、PK-15细胞组和Marc-145细胞组接种裸鼠后5周注射部位均形成肿块。对各处理组采集的肿块进行病理组织学检测，结果均为鳞状细胞癌细胞。对阳性对照BHK-21细胞组的一只裸鼠肺组织进行病理组织学检测发现有癌细胞的转移；阴性对照鸡胚成纤维细胞组、MDBK细胞组、OA3.Ts细胞组和ST细胞组小鼠均无肿块形成，接种后5周和12周分别进行剖检和病理组织学检测均未发现肿瘤细胞。由此可知，MDBK细胞、OA3.Ts细胞、ST细胞对裸鼠均不具有致瘤性；McCoy细胞、PK-15细胞、Marc-145细胞接种后可诱导裸鼠发生肿瘤，具有致瘤性；若使用致瘤性阳性细胞进行灭活疫苗生产时，应对灭活苗生产的工艺过程进行进一步的安全性评价。

Abstract:

In order to analyze the effect of six cell lines on the tumorigenicity of nude mice, 80 nude mice were randomly divided into 8 groups. The treatment group was divided into MDBK cells, OA3.Ts cells, Marc-145 cells, McCoy cells, PK-15 cells and ST cells. BHK-21 cells were used as positive control group and chicken embryo fibroblasts as negative control group. The treatment group and the control group are each injected into separate groups of 10 nude mice. After 5 and 12 weeks of feeding, the nude mice were dissected and histopathologically examined for tumor formation. The results showed that in BHK-21 cell group, McCoy cell group, PK-15 cell group and Marc-145 cell group, the nodule was formed at the injection site 5 weeks after inoculation in nude mice. Histopathological examination of the nodules collected from each treatment group showed that all the nodules were squamous cell carcinoma cells. Histopathological examination of lung tissue of a nude mouse with positive BHK-21 cells showed metastasis of cancer cells; there was no nodular formation in the chicken embryo fibroblast group, MDBK cell group, OA3.Ts cell group and ST cell group, and no tumor cells were found in 5 and 12 weeks after inoculation. These results suggested that MDBK cell group, OA3.Ts cell group and ST cell group had no tumorigenicity; McCoy cell group, PK-15 cell group and Marc-145 cell group could induce tumors in nude mice after inoculation，so they should be considered possibly tumourigenic. Production of inactivated vaccine with tumor positive cells should be further evaluated for the safety of the production process.