一测多评法测定白头翁口服液中3种生物碱成分研究

Simultaneous Determination of Three Alkaloid Ingredientsin Baitouweng Oral Solution by QAMS

关键词:  白头翁口服液  生物碱  一测多评

Key words:  Baitouweng oral solution  Alkaloid  Quantitative analysis of multi-components by single marker

摘要:

建立同时测定白头翁口服液中3种生物碱成分含量的一测多评法。采用Agilent ZORBAX SB C18(4.6×250mm,5μm)色谱柱分离,乙腈-0.05mol/L磷酸二氢钾溶液(50∶50)(每100ml中加十二烷基硫酸钠0.4g,再以磷酸调节pH为4.0)为流动相,流速为1.0 mL/min,采用347nm作为检测波长进行检测。以小檗碱为内参物,分别计算与黄连碱和巴马汀的相对校正因子及相对保留时间,实现一测多评。本研究拟为白头翁口服液质量控制提供新的思路。

Abstract:

To establish a method for simultaneous determination of three alkaloid ingredients in Baitouweng oral solution. An Agilent ZORBAX SB C18 column (4.6×250mm,5μm) was adopted as analytical column. The mobile phase consisted of Acetonitrile-0.05mol/L potassium dihydrogen phosphate solution (50∶50) (Add 0.4g of sodium dodecyl sulfate per 100ml, then adjust the pH to 4.0 with phosphoric acid) at a flow rate of 1.0 mL/min, the detection wavelengths was 347 nm. Taking berberine as an internal reference, the relative correction factors and relative retention time of coptisine and palmatine were calculated respectively. By measuring the content of one component, the simultaneous determination of the content of multiple components is achieved. This study is intended to provide new ideas for the quality control of Baitouweng oral solution.