海南霉素钠预混剂在鸡体内的药动学及生物利用度研究

Pharmacokinetics and bioavailability of Hainan mycomycin sodium premix in chickens

关键词:  海南霉素  药动学  生物利用度  鸡

Key words:  Hainanmycin  pharmacokinetics  bioavailability  chicken

摘要:

为研究海南霉素钠预混剂在鸡体内的药代动力学特征和生物利用度，将16只健康AA鸡随机分成2组，每组8只，采用平行试验设计对两组鸡分别进行单剂量口服给药和静脉注射给药药动学研究，给药量均为1.5 mg·kg -1 bw（相当于7.5 mg·kg -1混饲给药）。按预定时间点采集血样，血样中海南霉素的含量采用高效液相色谱-串联质谱法（HPLC-MS/MS）测定，流动相为乙腈-0.1％甲酸水溶液(90:10,V/V)。实测血药浓度-时间数据使用Winnonlin 5.2药动学分析软件拟合药动学参数。鸡口服给药的药动学参数如下：平均消除半衰期为（T1/2β）约为30.44 h，平均滞留时间（MRT）约为36.40 h，在血浆中的达峰时间(Tmax)约为0.5 h，达峰浓度（Cmax）约为 68.87 ng/ml，平均药时曲线下面积（AUC）约为 654.95 ng·h/ml，平均生物利用度（F）约为32.82％。鸡静脉注射给药的药动学参数如下：平均消除半衰期约为（T1/2β）为46.40 h，平均滞留时间（MRT）约为30.91 h，平均血浆清除率(CL)约为1.59 L/kg·h，平均表观分布容积（Vd）约为116.05 L/kg。结果表明海南霉素进入鸡体后分布广泛，消除缓慢，半衰期长；口服海南霉素钠预混剂吸收迅速，但吸收不完全。

Abstract:

To study the pharmacokinetics and bioavailability of Hainanmycin sodium premix in chickens,divided 16 healthy AA chickens into 2 groups randomly, and 8 in each group. The pharmacokinetics of single dose oral administration and intravenous administration in two groups of chickens were studied by parallel design,and the dose was 1.5 mg·kg -1 bw（Equivalent to 7.5 mg·kg -1 mixed feeding）.Collected blood samples according to the scheduled time，and the content of Hainanmycin in blood samples was determined by HPLC-MS/MS, and the mobile phase was acetonitrile-0.1％formic acid aqueous solution (90:10, V/V). The pharmacokineticp- parameters were fitted by Winnonlin 5.2 pharmacokinetic analysis softwar..The pharmacokinetic parameters of chicken oral administration were as follows: the mean elimination half-life (T1/2β) was 30.44 h，the mean resident time (MRT) was 36.40 h the peak time (Tmax) was 0.5 h, the peak concentration (Cmax) was 68.87 ng/ml ,the area under the mean drug time curve (AUC) was 318.93 ng·h/ml, the mean bioavailability (F) was 32.82％. The pharmac- okinetic parameters of chicken intravenous administration were as follows: the mean elimination half-life (T1/2β) was 46.40 h, the mean resident time (MRT) was 30.91 h，the mean plasma clearance (CL) was 1.59 L/kg·h,the mean apparent distribution volume was 116.05 L/kg.The results showed that the premix of Hainanmycin sodium absorbed rapidly, but not completely. After entering chicken body, Hainanmycin was widely distributed, eliminated slowly and had a long half-life.