HPLC法同时测定清瘟解毒口服液中栀子苷、黄芩苷的含量

The contents of Geniposide and Baicalin in Qingwen Jiedu Oral Liquid were determined simultaneously by HPLC

关键词:  清瘟解毒口服液  栀子苷  黄芩苷  HPLC

Key words:  Qingwen Jiedu Oral Liquid  Gardenoside  Baicalin  HPLC

摘要:

建立同时测定清瘟解毒口服液中栀子苷和黄芩苷含量的HPLC方法。采用十八烷基键合硅胶为填充剂,以0.2%磷酸溶液为流动相A,以乙腈为流动相B进行梯度洗脱,流速为1.0 mL/min,二极管阵列检测器,提取波长为238 nm,进样量为10 μL。试验结果表明,栀子苷在5~250 μg/mL(Y=14614X+35809,R²=0.9999),黄芩苷在10~500 μg/mL(Y=13270x+23503,R²=1.00)均呈良好的线性关系；两者平均回收率(n=6)分别为100.14%、99.42%,RSD分别为0.87%、0.94%。该方法操作简单,准确性、重复性、精密度、耐用性良好,适用于该制剂的质量控制,为制剂标准的完善提供了科学依据。

Abstract:

To establish a HPLC method for simultaneous determination of geniposide and baicalin in Qingwen Jiedu oral liquid. It was tested with C18 column, using 0.2% phosphoric acid solution as mobile phase A and acetonitrile as mobile phase B for gradient elution, flow rate was 1 mL per minute. Using photo diode array detector and the extract wavelength was 238 nm, Injection volume is 10 μL. The test results show that gardenoside and baicalin all had a good linear relationship in the range of 5~250 μg/mL(Y=14614X+35809, R²=0.9999) and 10~500 μg/mL(Y=13270x+23503,R²=1.00)respectively. The average recovery rate (n=6) was 100.14% and 99.42%, respectively, and the RSD was 0.87% and 0.94%, respectively. The method is simple to operate and has good accuracy, repeatability, precision and durability, which suitable for the quality control of the preparation, and provides a scientific basis for the improvement of the preparation standard.