| 摘要: |
| 通过性状、显微鉴别、薄层色谱鉴别，水分及总灰分的检查，醇溶性浸出物的测定，绿原酸和咖啡酸含量的HPLC同时测定初步建立了白英的质量标准。水分及总灰分不得大于7.0%和9.0%。醇溶性浸出物不得少于4.0%。绿原酸和咖啡酸含量的HPLC同时测定以C18柱为固定相，0.4%的磷酸溶液-乙腈（87: 13）为流动相，流速为1.0mL.min-1，柱温30℃，检测波长327nm。采用外标法定量计算含量。绿原酸的线性范围为5.0~1000μg.mL-1，回归方程为Area =14.924×Amt+2.6256 (n = 5)，相关系数r2= 0.99999。添加量为1.50mg.g-1的8个添加样品的平均回收率为86.5%；咖啡酸的线性范围为2.0~50μg.mL-1，回归方程为Area =24.423×Amt+2.4557 (n = 5)，相关系数r2= 0.99998。添加量为55μg.g-1的8个添加样品的平均回收率为83.0%。方法专属性强，灵敏度佳，准确度高，可作为控制白英的质量标准。 |
| 关键词: 质量标准 白英 含量 总灰分 醇溶性浸出物 绿原酸 咖啡酸 高效液相色谱法 |
| DOI： |
| 投稿时间：2020-04-19修订日期：2020-10-16 |
| 基金项目:兽药国家标准制修订项目（2017Z001）；贵州省科技计划项目(黔科合支撑[2019]2320号) |
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| Preliminary Study on the Quality Standard of Solanum lyratum Thunb |
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| (Guizhou Provincial Supervisory Institute of Veterinary Drug and Feeds,Guiyang) |
| Abstract: |
| A quality standard for Solanum lyratum Thunb has been developed to identify Solanum lyratum Thunb by microsope and TLC, to test contents of moisture, total ashes and ethanol extracts, to determine contents of chlorogenic acid and caffeic acid in it by HPLC as well. Acording to this quality standard, the contents of water and total ashes should be more than 7.0% and 9.0%, while that of ethanol extracts should not less than 4.0% in Solanum lyratum Thunb. A method is developed to Simultaneously determine the content of chlorogenic acid and caffeic acid in Solanum lyratum Thunb by HPLC with C18 column as stationary phase, a solution of 0.4%?phosphoric solution and acetonitrile = 87 : 13 as mobile phase，flow rate of 1.0 mL·min-1, column temperature of 30 degree Celsius, and wavelength of 327nm. It is quantified by external standard method. The linear range of chlorogenic acid is between 5.0 and 1000 mg.mL-1, and the regression equation is Area = 14.924×Amt+2.6256(n = 5), r2 =0.99999. Result shows that the average recovery of 8 samples of Solanum lyratum Thunb is 86.5% when added 1.50mg.g-1 of chlorogenic acid to each. While the linear range of caffeic acid is between 2.0 and 50.0 μg.mL-1, and the regression equation is Area = 24.423×Amt+2.4557(n = 5), r2 =0.99998. Result shows that the average recovery of 8 samples of Solanum lyratum Thunb is 83.0% when added 55μg.g-1 of caffeic acid to each. Methods in the quality standard are precise, specific and accurate methods, could be used for controlling the quality of Chinese medicine Solanum lyratum Thunb. |
| Key words: quality standard Solanum lyratum Thunb content total ashes ethanol-soluble extractives chlorogenic acid caffeic acid HPLC |
