4型禽腺病毒HLJ1701株灭活疫苗的研制

Development of Inactivated Avian Adenovirus Type 4 HLJ1701 Strain

关键词:  4型禽腺病毒  灭活疫苗  佐剂  研制  安全性  保护率

Key words:  avian adenovirus type 4  inactivated vaccine  adjuvant  development  safety  protection rate

摘要:

利用4型禽腺病毒HLJ1701株进行灭活疫苗的研制,并对疫苗的免疫效果进行评价,为家禽4型禽腺病毒的防控提供数据及参考。将HLJ1701株用灭菌生理盐水作10⁴倍稀释后,接种9日龄SPF鸡胚,37℃孵育72ｈ后收获感染鸡胚尿囊液,经甲醛灭活后,加白油佐剂乳化制成油乳剂灭活疫苗,对制备疫苗的性状、安全性、免疫效力等进行检验。结果显示,制备的3批4型禽腺病毒灭活疫苗(HLJ1701株)均为油包水型,黏度均在50 cP以内,对3批疫苗取样,样品经3000 rpm/min离心15 min,管底无水相析出。安全性试验结果显示,将疫苗按1 mL/只超剂量接种3周龄SPF鸡,试验鸡在观察期内全部健活,未出现局部或全身不良反应,表明疫苗对SPF鸡具有良好的安全性；免疫效力及攻毒保护试验结果显示,用疫苗按0.2 mL/只的剂量免疫接种3周龄SPF鸡1次,免疫接种后21ｄ 试验鸡血清中HLJ1701株的抗体平均效价可达2⁸以上,使用4型禽腺病毒(HLJ1701株)接种0.2 mL/只(100 LD50)对免疫鸡进行攻毒,疫苗对免疫鸡的保护率均为100％。在实验室条件下研制出4型禽腺病毒(HLJ1701株)灭活疫苗,疫苗的各项指标均符合标准。

Abstract:

Inactivated vaccine was developed by type 4 avian adenovirus HLJ1701 strain, and the immune effect of the vaccine was evaluated to provide data and reference for the prevention and control of poultry type 4 avian adenovirus. The HLJ1701 strain was diluted 10⁴ times with sterilized physiological saline, and 9-day-old SPF chicken embryos were inoculated, and the allantoic fluid of infected chicken embryos was harvested after incubation at 37 ° C for 72 hours. After being inactivated by formaldehyde, white oil adjuvant was added to emulsify to make oil. The vaccines are used to test the characteristics, safety, and immune efficacy of the vaccine. The results showed that the three batches of type 4 inactivated avian adenovirus vaccine (HLJ1701 strain) were all w/o and the viscosity was within 50 cP. The samples were centrifuged at 3000 rpm / min for 15 minutes. The bottom anhydrous phase precipitated. The results of the safety test showed that the vaccine was inoculated with SPF chicken at an overdose of 1 mL per animal. The test chickens were all alive during the observation period without local or systemic adverse reactions, indicating that the vaccine has good safety to SPF chickens. The immune efficacy and challenge protection test results show that the vaccine was used to vaccinate 2-week-old SPF chickens once at a dose of 0.2 mL per animal. The average titer of the antibody against HLJ1701 strain in the serum of test chickens reached 2⁸Type 4 avian adenovirus (HLJ1701 strain) was inoculated with 0.2 mL (100 LD50) to challenge the immunized chickens, and the protection ratio of the vaccine to the immunized chickens was 100%. An inactivated vaccine against avian adenovirus type 4 (HLJ1701 strain) was developed under laboratory conditions, and all indexes of the vaccine met the standards.