连花柴芩可溶性粉的毒理学研究

Study on Toxicology of Lianhuachaiqin soluble powder

关键词:  连花柴芩可溶性粉  急性毒性  长期毒性

Key words:  lianhuachaiqin soluble powder  acute toxicity  chronic toxicity

摘要:

研究连花柴芩可溶性粉的急性毒性与长期毒性，评价其临床用药的安全性，为临床用药提供科学依据。急性毒性实验以预实验的最高剂量组25.0 g·kg-1，设为试验高剂量组进行实验。长期毒性实验分为四个组，即试验高、中、低剂量组（剂量分别为25.0 g·kg-1，12.5 g·kg-1，6.25 g·kg-1）与空白对照组，每组大鼠20只，雌雄各半，每周给药7 d，连续给药30 d，观察一般症状、体重、摄食量、血液学、血液生化学及脏器系数，并作组织病理学检查。结果显示，急性毒性实验中小鼠灌胃给药后14 d内未见小鼠死亡，亦未见明显毒性反应，口服连花柴芩可溶性粉LD50>25.0 g·kg-1。按照毒理学急性毒性剂量分级表，口服LD50>5 000 mg·kg-1时为实际无毒。长期毒性试验中连花柴芩可溶性粉对SD大鼠灌胃给药，所有检测指标未出现因给予受试物而引起相关的毒性变化，且未发现连花柴芩可溶性粉的目标脏器。综上连花柴芩可溶性粉口服给药具有较好的安全性。

Abstract:

Investigate the acute and chronic toxicity of lianhuachaiqin soluble powder, evaluate the safety of clinical medication, provide scientific basis for clinical medication. Maximum dose group of preliminary experiment (25.0 g·kg-1) was set as high dose group of experiment in acute toxicity experiment. Long-term toxicity test was divided into high, medium, low dose group and blank control group, doses of medication were 25.0 ,12.5 and 6.25 g·kg-1, each group of 20 rats, male and female half, continuous administration once a day for 30 days, observe the general symptoms, weight, food intake, hematology, blood biochemistry and viscera coefficient, and histopathological examination had done.The results showed that no rats died within 14 days after intragastric administration in the acute toxicity test, without obvious toxic reaction, and LD50 of lianhuachaiqin soluble powder was 25.0 g·kg-1 in oral medication. According to the toxicology acute toxicity dose scale, oral LD50>5000 mg·kg-1 is actually non-toxic. In the long-term toxicity test, no related toxicity changes was found of all test indexes caused by administration of tested substance, and no target organs of soluble powder was found. In conclusion, lianhuachaiqin soluble powder is safety with oral administration.