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别隐品碱和原阿片碱在猪肝S9的代谢研究
赵雪娇,黄亚军,张明军,刘兆颖
0
(湖南农业大学)
摘要:
试验采用高效液相色谱/四极杆飞行时间质谱(HPLC-QqTOF-MS)技术,进行别隐品碱(ALL)和原阿片碱(PRO)在猪肝S9中的代谢研究。通过比较这些代谢物的精确分子量和产物离子与前体离子或代谢物的变化,进行了这些代谢物的结构鉴定。结果显示,在猪肝S9中检测到低浓度的ALL和PRO,分别鉴定出4种ALL和3种PRO的代谢物,O-去甲基和C2-C3位亚甲二氧环环裂后去甲基分别为ALL和PRO是猪肝体外的主要代谢途径,且2,3-亚甲二氧环去甲基比9,10-亚甲二氧环去甲基更占优势。其研究结果可为解释博普总碱的药动学和食品安全性评价提供理论依据。
关键词:  HPLC-QqTOF-MS  别隐品碱  原阿片碱  猪肝  代谢
DOI:
投稿时间:2020-03-11修订日期:2020-06-19
基金项目:国家重点研发计划项目(2017YFD0501503)
Metabolism of allocryptopine and protopine in S9 of pig liver
(Hunan Agricultural University)
Abstract:
High performance liquid chromatography / quadrupole-time-of-flight (HPLC-QqTOF-MS) mass spectrometry method was used to study the metabolism of allocryptopine(ALL) and protopine(PRO) in pig liver S9. The structure of these metabolites was identified by comparing the exact molecular weight of these metabolites and the changes of product ions with precursor ions or metabolites. The results showed that Metabolites of 4 ALL and 3 PRO were detected in S9 of pig liver. It can be concluded that O-demethylation followed by ring-cleavage and demethylation at C2-C3 are ALL and PRO, respectively, the most important metabolic pathways in vitro. Moreover, 2,3-methoxycyclodemethylation is more advantageous than 9,10-methoxycyclodemethylation. The results can provide theoretical basis for the pharmacokinetic and food safety evaluation of Macleaya alkaloids.
Key words:  HPLC-QqTOF-MS  allocryptopine  protopine  pig liver  metabolism

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