中药精液消毒剂的消杀效果及安全性评价研究

Study on the Germicidal Efficacy and Safety Evaluation of a Chinese Medicine Semen Disinfectant

关键词:  中药精液消毒剂  鸡精液  病原微生物  杀灭效果  安全性评价

Key words:  Chinese medicine semen disinfectant  chicken semen  pathogenic microorganism  killing effect  safety evaluation

摘要:

为了解新研制的中药精液消毒剂的消毒效果和安全性，本研究开展了体外抑菌杀毒和安全性综合评价实验。体外药效学实验显示：该消毒剂对大肠杆菌高敏，对门氏菌、禽巴氏杆菌和金黄色葡萄球菌均中敏，4种菌的体外MIC值分别为31.3、62.5、62.5和31.3 mg/mL；消毒剂按1∶400及以上浓度与新城疫病毒互作，可在5 min内对该病毒有100%杀灭率。安全性评价实验显示：该消毒剂的小鼠急性毒性LD50值＞5000 mg/Kg，无毒性；对兔子的皮肤和眼睛染毒，无刺激；消毒剂按1∶10浓度及以下，对常温稀释保存的种公鸡精液中精子生存指数影响不大。临床应用实验显示：在冻融条件下，该消毒剂1∶10浓度组的种公鸡精液中精子存活率要显著高于其它试验组(P＜0.01)；消毒剂按1∶10浓度对种公鸡精液稀释消毒，种蛋受精率和孵化率都有明显提高，但差异不显著(P＞0.05)。结果表明：该消毒剂不仅对种公鸡精液中常见病原微生物具有较好的杀灭效果，而且安全无刺激、无毒，对种鸡繁育无不良影响。

Abstract:

In order to know the disinfection effect and safety of the newly developed Chinese traditional medicine semen disinfectant, the comprehensive evaluation and disinfection experiments in vitro was carried out.The tests of pharmacodynamics in vitro showed that the disinfectant was highly sensitive to E.coli,moderately sensitive to Salmonella, Avian pasteurella and Staphylococcus aureus.The MIC values of four strains were 31.3, 62.5, 62.5 and 31.3 mg/mL, respectively.The kill rate of the disinfectant at a concentration of 1∶400 or above to Newcastle disease virus in 5 minutes was 100%. The results of safety evaluation showed that the LD50 of the disinfectant was over 5000 mg/kg in acute toxicity in mice, and had no toxicity to the skin and eyes of rabbits.The disinfectant had little effect on the survival index of sperm in the semen of breeder roosters preserved at room temperature.The results of clinical application showed that the survival rate of sperm in 1∶10 concentration group was significantly higher than that in other test groups (P<0.01),and the sperm was diluted and disinfected at 1∶10 concentration, the fertilization rate and hatch-ability of seed eggs were significantly increased, but the difference was not significant (P>0.05).The results performed that the disinfectant not only had a good killing effect on the common pathogenic microorganisms in the semen of breeder roosters, but also was safe, non-irritating, non-toxic and had no adverse effect on breeding.