一株鸡传染性贫血病毒的分离鉴定及其致病性研究

Isolation and Identification of a Chicken Infectious Anemia Virus and Study on Its Pathogenicity

关键词:  鸡传染性贫血病毒  致病性  序列分析

Key words:  chicken infectious anemia virus  pathogenicity  sequence analysis

摘要:

从北京某鸡场发生疑似鸡传染性贫血病毒（CAV）感染的鸡只病料中分离到了一株CAV，通过PCR和全基因组测序等方法对其进行了鉴定，命名为AV1550。将其全基因组序列与NCBI上的参考毒株序列进行同源性比对和进化分析，结果显示AV1550株与CAV参考毒株的同源性在91.7%~99.7%，与中国分离株LN15170的亲缘关系最近。VP1序列分析表明AV1550在 75、89、125、141和394位氨基酸均为强毒株特征。1日龄SPF鸡经胸部肌肉途径接种含10000EID50的AV1550病毒液后，接种鸡只出现明显的贫血症状，增长迟缓，死亡率高达50%，表明AV1550是一株具有较强致病性的CAV野毒株。

Abstract:

A CAV strain, named AV1550, was isolated from suspected CAV infection in a chicken farm in Beijing and identified by PCR and whole genome sequencing. Homology comparison and evolutionary analysis were conducted with the reference strain sequences on NCBI, the result showed that the homology between AV1550 strain and CAV reference strains was 91.7%~99.7%, and it was closest to the Chinese isolate LN15170. Besides, AV1550 was of virulent characteristic amino acids at the position of VP1 75, 89, 125, 141 and 394.The dose of 10000EID50 virus was inoculated with 1-day-old SPF chickens through chest muscles, causing significant anemia and growth retardation, and the mortality reached as high as 50%, indicating that AV1550 was a wild CAV strain with strong pathogenicity.