HPLC-PDA法测定兽药中非法添加的喹乙醇、乙酰甲喹检查方法

Determination of Olaquindox and Mequindox Illegally Added in Twelve Kinds of Veterinary Drug by HPLC-PDA

关键词:  喹乙醇  乙酰甲喹  兽药  HPLC-PDA

Key words:  Olaquindox  Mequindox  Veterinary drug  HPLC-PDA

摘要:

建立兽药中非法添加喹乙醇、乙酰甲喹的通用检查方法。以十八烷基键合硅胶为填充剂,0.01 mol/L磷酸二氢钾溶液-乙腈为流动相,梯度洗脱,流速为1.0 mL/min,波长扫描范围为200-400 nm,柱温30℃,并采用峰纯度检查和光谱相似度检查辅助对照品比对方法,对非法添加药物进行确证。结果显示,喹乙醇、乙酰甲喹与其他物质峰分离良好；喹乙醇和乙酰甲喹在12种兽药中的平均回收率分别为96.6%~101.4%和98.0%~101.3%,RSD均小于1.4%；喹乙醇和乙酰甲喹的检测限均为1.0 g/kg(L)。该检查方法简便、准确、可靠,可用于检查兽药中非法添加的喹乙醇和乙酰甲喹。

Abstract:

A method for the determination of olaquindox and mequindox illegally added in twelve kinds of veterinary drug was developed by the high performance liquid chromatography with photo-diode array detector (HPLC-PDA). It was tested with C18 column, using gradient elution with 0.1% potassium dihydrogen phosphate and acetonitrile as the mobile phase. The extract wavelength was 365 nm. Peak purity test and spectrum similar test were help to determine the olaquindox and mequindox except the method of comparison between sample and reference. The mean recovery of olaquindox and mequindox were between 96.6%~101.4% and 98.0%~101.3% respectively, and RSD were less than 1.4%；The detection limits of olaquindox and mequindox were both 1.0 g/kg(L). In conclusion, the method is simple, accurate and reliable for the determination of olaquindox and mequindox in veterinary drug.