新肝宝对鲶爱德华氏菌感染黄颡鱼的保护作用研究

Study on the Protective Effect of Xin Gan Bao on Yellow Catfish Pelteobagrus fulvidraco Infected with Edwardsiella ictaluri

关键词:  新肝宝  黄颡鱼  鲶爱德华氏菌

Key words:  Xin Gan Bao  yellow catfish Pelteobagrus fulvidraco  Edwardsiella ictaluri

摘要:

为了考察新肝宝对鲶爱德华氏菌感染黄颡鱼的保护作用,采用不同粉碎粒度的新肝宝拌饲投喂7d,随后用鲶爱德华氏菌腹腔注射攻毒,观察死亡率、酶学指标及组织病理变化。结果显示,新肝宝组死亡率(48.57%和44.28%)低于对照组(54.29%),溶菌酶及超氧化物歧化酶活性显著高于对照组(p＜0.05),肝脏、肾脏、脾脏损伤情况均小于对照组,其中600目作用效果优于200目。表明,新肝宝能够在一定程度上减轻鲶爱德华氏菌感染导致的肝、肾、脾损伤,提高黄颡鱼非特异性免疫力,增强黄颡鱼对鲶爱德华氏菌的抵抗能力,其中600目作用效果优于200目。

Abstract:

The study was aimed to evaluate the protective effect of Xin Gan Bao on yellow catfish( Pelteobagrus fulvidraco) infected with Edwardsiella ictaluri. The fish were intraperitoneally injected with Edwardsiella ictaluri after feeded with diet containing Xin Gan Bao of 200-mesh and 600-mesh for 7 days. Then the mortality, enzyme activities and histopathology changes were investigated. The results showed that mortalities of Xin Gan Bao groups(48.57% and 44.28%) were lower than that of the control group(54.29%). The levels of lysozyme(LZM) and superoxide dismutase(SOD) were higher than that of the control group significantly(p＜0.05). The damageSofSliver,Skidney and spleen were lighter than the control group. And Xin Gan Bao of 600-mesh had better effect than the 200-mesh. It indicated that Xin Gan Bao could reduce the damage caused by Edwardsiella ictaluri infection, improve the non-specificSimmunity and resistance to Edwardsiella ictaluri of yellow catfish. And Xin Gan Bao of 600-mesh had better effect than the 200-mesh.