3种不同类型佐剂的猪圆环病毒2型亚单位灭活疫苗免疫效果的比较研究

Comparative Study on the Immune Effect of the Porcine Circovirus Type 2 Subunit Vaccines Prepared with Three Different Types of Adjuvants

Key words:  adjuvants, porcine  circovirus type 2, subunit, inactivated  vaccine, immune  effect

摘要:

为制备猪圆环病毒2型商品化亚单位疫苗选择良好的佐剂，对由水性佐剂GEL 01、白油佐剂以及氢氧化铝胶佐剂分别制备的3种猪圆环病毒2型亚单位疫苗的免疫效果进行比较研究。本实验选取3~4周龄猪圆环病毒2型抗体阴性猪30头，根据试验疫苗接种情况分为6组，分别为：3种不同佐剂制备的亚单位疫苗（A组、B组和C组）、商品化疫苗（D组）以及空白对照组和攻毒对照组，然后进行临床观察、抗体水平监测和免疫效力评价，观察各组仔猪的不良反应情况、抗体水平以及攻毒保护情况。结果显示：3种不同类型佐剂制备的猪圆环病毒2型亚单位疫苗接种猪后均无任何不良反应、能刺激机体产生良好的ELISA抗体水平和免疫效果，但水性佐剂GEL 01制备的疫苗与另外2种佐剂制备的疫苗相比，综合抗体水平、免疫效果等因素，水性佐剂GEL 01制备的疫苗在临床使用上更具优势，为商品化亚单位疫苗佐剂的选择提供重要的数据支持。

Abstract:

In order to select a good adjuvant to prepare the commercial subunit vaccine of porcine circovirus type 2 (PCV 2) , the immune effects of three kinds of PCV 2 subunit vaccines prepared by water adjuvant gel 01, white oil adjuvant and aluminum hydroxide gel adjuvant were compared. In this experiment, according to the vaccination conditions, thirty 3-4 weeks-old pigs with seronegative for PCV2 antibodies were divided into 6 groups. They were the group A to group C for the subunit vaccines prepared with three different adjuvants separately, group D for the commercial vaccines, and the control group and the attack control group. Then, the clinical observation, antibody level monitoring and immune efficacy evaluation were carried out, and the adverse reactions, antibody levels and the protection of the piglets were observed. The results show that there were no adverse reactions after vaccination of swine circovirus type 2 subunit vaccine prepared by three different types of adjuvants, which could stimulate the body to produce good ELISA antibody level and immune effect. Feurthermore , compared with the vaccines prepared with the other two adjuvants, the vaccines prepared with gel 01 showed better antibody level, immune effect and more advantages in clinical applications. So, it provides important data support for the selection of adjuvants for commercial subunit vaccines.