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实验动物粪便中金黄色葡萄球菌和沙门菌检验方法的比较
王秀丽,辛凌翔、李建、张一帜,张媛,李俊平
0
(中国兽医药品监察所)
摘要:
为了探讨实验动物粪便中金黄色葡萄球菌和沙门菌的检测方法,试验比较了国标的方法、ATB生化鉴定试纸条、16s rRNA测序分析对样品进行检测,结果表明生化鉴定试纸条和16s rRNA测序分析均可以准确、高效地检测出沙门菌和金黄色葡萄球菌,而参考国标的方法,有些金黄色葡萄球菌和沙门菌的鉴定结果与国标不一致。说明用ATB生化鉴定试纸条和16s rRNA序列分析检测沙门菌和金黄色葡萄球菌及其他菌的方法更可靠。本研究可以为国家标准和行业标准的修订提供参考数据。
关键词:  金黄色葡萄球菌,沙门菌,检测
DOI:
投稿时间:2019-11-04修订日期:2019-12-07
基金项目:
Compare of the detection methods of Staphylococcus aureus and Salmonella in experimental Animals’
(China Institute of Veterinary Drug Control Beijing 100081)
Abstract:
National standard method, ATB biological identification card and 16s rRNA sequence analysis were compared in the test of Staphylococcus aureus and Salmonella in experimental Animals’excrement, in roder to study the detection methods. The compare results showed that Staphylococcus aureus and Salmonella in excrement could be detected effectively and exactly in both of ATB biological identification method and 16s rRNA sequence analysis methods. But according to the national standards, some of the Staphylococcus aureus and Salmonella couldn’t be detected, showing that ATB biological identification method and 16s rRNA sequence analysis are reliable. This study can provide some reference data for the revise of national standards and professional standards.
Key words:  staphylococcus  aureus salmonella  detection

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