CpG ODN重组质粒对猪外周血淋巴细胞免疫刺激作用的研究

Immunostimulatory effect of CpG ODN recombinant plasmid on peripheral blood lymphocytes of pigs

关键词:  CpG ODN  重组质粒  佐剂

Key words:  CpG ODN  recombinant plasmids  adjuvants

摘要:

为获得对猪具有免疫刺激活性的含CpG序列的重组质粒,设计合成了11条CpG序列,应用MTS比色法测定合成CpG ODNs刺激猪PBMC增殖的能力,结果有10条CpG ODN对猪PBMC具有刺激活性(SI>2)；以对猪PBMC具有较高刺激活性的CpG06和CpG08为核心,分别合成含6次重复序列的重组质粒pCpG06和pCpG08,并检测其对猪PBMC的刺激活性,结果重组质粒pCpG06和pCpG08对猪PBMC的刺激指数分别可达4.97和5.32,并可刺激猪PBMC产生较高水平的IL-4。本研究获得的重组质粒pCpG06和pCpG08对猪PBMC具有较好的刺激活性,可为猪用CpG佐剂的研究提供参考。

Abstract:

To obtain the CpG recombinant plasmid with immunostimulatory activity against pig. Eleven DNA sequences containing different CpG motifs were designed and synthesized. MTS colorimetric assay was used to determine the ability of CpG ODNs to stimulate the proliferation of porcine PBMC. The result showed that ten CpG ODNs has the stimulating activity to the PBMC of porcine(SI>2). Recombinant plasmids pCpG06 and pCpG08 containing 6 repeats of CpG06 or CpG08 were synthesized, and tested for stimulating activity on porcine PBMC. The results showed that the SI value of recombinant plasmids pCpG06 and pCpG08 was 4.97 and 5.32, respectively, and the recombinant plasmids stimulated higher levels of IL-4 in porcine PBMC. The recombinant plasmids pCpG06 and pCpG08 obtained in this study have good immunostimulatory activity on porcine PBMC, which can provide a reference for the study of CpG adjuvants for pigs.