复方中草药与鼠李糖乳杆菌共发酵产物对鸡球虫的治疗效果研究

Study on the therapeutic effect of chinese herbal formula and Lactobacillus rhamnosus co-fermentation product on chicken coccidiosis

关键词:  球虫病  鼠李糖乳杆菌  发酵  抗球虫指数  中药

Key words:  coccidiosis  Lactobacillus rhamnosus  Ferment  ACI  herbal medicine

摘要:

为探究复方中草药与鼠李糖乳杆菌(Lactobacillus rhamnosus, L. rhamnosus)共发酵产物对鸡球虫病的治疗效果,本研究将复方中草药(青蒿、常山、白头翁、仙鹤草、槐花、黄芩、甘草)与L. rhamnosus共发酵12h,患球虫病雏鸡灌服共发酵产物,连续用药5日,观察雏鸡临床症状,并检测各组雏鸡的相对增重率,盲肠病变记分,盲肠病理切片,每克粪便卵囊值(OPG),并计算各组药物抗球虫指数(ACI)。结果显示,服用复方中药发酵制剂后的感染球虫的雏鸡体重显著上升,与对照组差异显著(P＜0.05)。此外,发酵产物可以明显减轻盲肠病理变化,同时可以降低受感染雏鸡卵囊的排出量。发酵产物组的ACI为130.59,达到中等抗球虫水平。复方中草药与L. rhamnosus共发酵提高了中药对鸡球虫病的治疗效果,为益生菌中药发酵治疗球虫病奠定基础。

Abstract:

In order to explore the therapeutic effect of the chinese herbal formula and L. rhamnosus co-fermentation product on chicken coccidiosis, the chinese herbal formula(QingHao, ChangShan, BaiTouWeng, XianHeCao, KuiHua, HuangQin, GanCao) and L. rhamnosus After 12 hours of fermentation, chicks suffering from coccidiosis were fed with co-fermentation products for 5 consecutive days. The clinical symptoms of chicks were observed, and the relative weight gain rate, cecal lesion scores, cecal pathological sections, oocysts per gram (OPG) per gram of chicks, and anticoccidial index (ACI) of each group were calculated. The results showed that the weight of the chicks infected with coccidia after taking the chinese herbal formula fermentation preparation increased significantly, and the difference was significant (P<0.05). In addition, the chinese herbal formula fermentation preparation can significantly reduce the pathological changes of the cecum, and at the same time reduce the discharge of the oocysts of the infected chicks. The ACI of the fermentation product group was 130.59, reaching a medium anticoccidial level. The co-fermentation of chinese herbal formula and L. rhamnosus improves the therapeutic effect of traditional Chinese medicine on chicken coccidiosis, and lays a foundation for the fermentation of probiotics to treat coccidiosis.