绵马贯众药材的快速多信息薄层鉴别方法研究

Study on Rapid Multi-information identification Method of Thin-layer

关键词:  绵马贯众  多信息  薄层色谱法

Key words:  dryopteridis crassirhizomatis rhizoma  multi-information  thin-layer chromatography

摘要:

为建立快速多信息薄层鉴别方法，采用薄层色谱，分别以三氯甲烷-乙酸乙酯-浓氨试液（10∶1∶0.1）、环己烷-乙酸乙酯-甲酸（12∶2∶0.2）、三氯甲烷-乙酸乙酯-甲醇-浓氨试液（4∶2∶4∶0.5）和三氯甲烷-乙酸乙酯-甲醇-浓氨试液（1∶2∶4∶0.5）为展开剂，通过紫外光灯365 nm、254 nm、日光和灯光检视，共检出绵馬贯众脂溶性、中极性和水溶性成分斑点18个。该方法简便、快捷、实用，可多指标、快速控制绵马贯众药材的质量，辨别其真伪。

Abstract:

In order to establish a rapid and multi information identification method, a TLC method was used, a mixture of trichloromethane，ethyl acetate and strong ammonia solution（10∶1∶0.1）, cyclohexane，ethyl acetate and formic （12∶2∶0.2）, trichloromethane，ethyl acetate, methanol and strong ammonia solution（4∶2∶4∶0.5）and chloroform, ethyl acetate, methanol, concentrated ammonia（1∶2∶4∶0.5）were used as the developer solution respectively, spots of dryopteridis crassirhizomatis rhizoma were viewed under UV 365nm, UV 254nm ,the daylight and the lights respectively . A total of 18 component spots of the liposoluble, the intermediate polar and water-soluble from Dryopteridis Crassirhizomatis Rhizoma were observed. This method is simple, rapid and practical, which could control the quality of the medicinal materials quickly and identify the authenticity.