长效土霉素注射液的急性毒性及肌肉刺激性研究

Acute toxicity and muscle irritation of long acting oxytetracycline injection

关键词:  长效土霉素注射液  急性毒性  局部刺激性

Key words:  long-acting oxytetracycline injection  acute toxicity  local irritation

摘要:

为了考察新型长效土霉素注射液的安全性；本文分别对小白鼠和家兔进行了急性毒性试验和局部刺激性试验。随机将60只小白鼠分为6组，每组10只，其中雌雄各半，进行急性毒性试验考察；随机将9只家兔分为3组，每组3只，进行局部刺激性试验。结果表明，该新型长效土霉素注射液小鼠肌肉注射LD50为600.32mg/kg，LD50的95%的可信区间为（600.32±2.44）mg/kg；随着时间延长和给药剂量增加，刺激性增强，注射长效土霉素7d后家兔股四头肌出现红肿、发紫、坏死、光泽消失现象，15d后肌肉组织的坏死范围扩大。表明该产品对小白鼠表现为低毒，对家兔的股四头肌有局部刺激性。

Abstract:

In order to investigate the safety of the new long-acting oxytetracycline injection, acute toxicity test and local irritation test were carried out on mice and rabbits respectively. Sixty mice were randomly divided into six groups, 10 mice in each group, half male and half female, and acute toxicity test was carried out. Nine rabbits were randomly divided into three groups with 3 rabbits in each group for local stimulation test. The results showed that the LD50 of the new long-acting oxytetracycline injection was 600.32mg/kg, and the 95% confidence interval of LD50 was (600.32 ± 2.44) mg / kg. With the prolongation of time and the increase of dosage, the irritation increased. The swelling, purple, necrosis and gloss disappeared in quadriceps femoris muscle of rabbits 7d after injection of long-acting oxytetracycline, and the necrosis area of muscle tissue expanded 15d later.The results showed that the product had low toxicity to mice and local irritation to rabbit quadriceps femoris.