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苦参功劳颗粒中盐酸小檗碱含量测定研究
杨会鲜,姚路路,周冰,张聪,周德刚
0
(洛阳惠中兽药有限公司)
摘要:
建立并验证了苦参功劳颗粒中盐酸小檗碱的含量测定方法。采用高效液相色谱法,色谱柱为Agilent Zorbax SB-C18(250 × 4.6 mm,5 μm),流动相为乙腈-0.05mol/L磷酸二氢钾缓冲液;检测波长为265 nm;流速为1.0 ml/min;柱温为30 ℃。试验结果表明盐酸小檗碱在4.9~78.6μg.ml-1范围内,峰面积与浓度呈良好的线性关系(r = 1),加样回收率在101.38%-103.74%之间,平均回收率为102.7%, RSD=0.8%(≤2.0%)。所建立的方法简便、准确度高、专属性强,可用于苦参功劳颗粒中盐酸小檗碱的含量测定。
关键词:  苦参功劳颗粒  盐酸小檗碱  高效液相色谱法  含量  十大功劳
DOI:
投稿时间:2019-09-26修订日期:2019-12-25
基金项目:国家科技攻关计划
Determination of Berberine Hydrochloride in Kushen Gonglao Granules
(Luoyang Huizhong Animal Medicine Co.,Ltd)
Abstract:
A method for determining the content of berberine hydrochloride in Kushen Gonglao Granules was established and verified. Using high performance liquid chromatography, the column was Aglient Zorbax SB-C18 (250 × 4.6 mm, 5 μm), and the mobile phase was acetonitrile-0.05mol/L potassium dihydrogen phosphate buffer; the detection wavelength was 265 nm; the flow rate was 1.0. ml/min; column temperature is 30°C.The results showed that the berberine hydrochloride injection concentration ranged from 4.9 to 78.6μg.ml-1, and the peak area showed a good linear relationship (r=1). The recovery test was between 101.38% and 103.74%. The recovery test was 102.7%, and RSD was 0.8% (≤2.0%). The established method is simple, accurate and specific, and can be used for the determination of berberine hydrochloride in Kushen Gonglao Granule.
Key words:  Kushen gonglao granule  Berberine hydrochloride  HPLC  content  mahoniae folium

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