氯霉素类检测前处理方法和残留现状探讨—以湖南某市畜禽肉为例

Discussion of determination pretreatment method and residues of chloramphenicols—Taking livestock and poultry meat collected from a city in Hunan province as examples

关键词:  畜禽肉  氯霉素类  前处理方法

Key words:  Livestock and poultry  Chloramphenicols  Pretreatment method

摘要:

摘要：确定畜禽肉氯霉素类残留量测定方法中最佳前处理方法，初步得出氯霉素类残留现状。基于已报道方法进行前处理方法比较；用新建立的UPLC-MS/MS方法对不同品种、月份和区域样本进行含量测定；借助Excel 2010和SPSS 18.0对测定结果进行整理和两两比较分析。前处理方法比较研究中，最佳提取溶剂为乙腈，且无需过柱净化；检测结果中，氯霉素为未检出，氟苯尼考检出率为16.1%，且63.3%的样品检出值为0.1~1.0 μg/kg；残留现状分析中，3、4、5月，猪肉和鸡肉，东区和西区残留最为严重。新复极差法分析显示两两间比较并无显著性差异（P>0.05）。本实验方法简便可靠，可用于畜禽肌肉组织中氯霉素类残留量的检测，残留现状分析对监督者具有指导作用。

Abstract:

To determine the best pretreatment method?for determination of chloramphenicols and obtain the rule of residues in muscle tissue of livestock and poultry. The best pretreatment method was obtained based on the comparison of published methods. The newly established UPLC- MS/MS method was used to test the content of chloramphenicols in samples which were?classified based on different form included varieties, months and regions. The results were sored out and compared with each other by Excel 2010 and SPSS 18.0. As a result, the best extraction solvent was acetonitrile, and the column purification was not neccessary. The determination results showed that chloramphenicol did not have been detected with the detection rate of 16.1% of the florfenicol, and the detection?value of the florfenicol in 63.3% samples was between 0.1 μg/kg and 1.0 μg/kg. Analysis of the situation of residues showed that the degree of residual of the pork and chicken, eastern and west district and the month included March, April and May were the highest. There was no significant difference (P>0.05) among these results based on the analysis result of Duncan，S. At last, this method was simple and reliable, and could be used for the detection of chloramphenicols residues in muscular tissue of livestock and poultry. Moreover, analysis of the situation of residues showed a strong guiding effect for the regulator.