2019年我国部分地区鸭圆环病毒基因序列测定与分析研究

Sequence Analysis and Analysis of Duck Circovirus Genes in Some Regions of China in 2019

关键词:  鸭圆环病毒  全基因克隆  序列分析

Key words:  duck circovirus  complete genome  sequence analysis

摘要:

为了解近年鸭圆环病毒流行毒株的基因遗传特点和变异情况，通过采用PCR的方法对我国部分地区的30份鸭圆环病毒阳性样品进行全基因组扩增与克隆、序列测定及遗传进化分析。结果显示：30株DuCV流行毒株之间的全基因序列同源性为85.8%~99.9%，其中26株DuCV的全基因序列长为1993~1995 bp，与德国代表株处于同一进化分支，属于基因1型，4株DuCV的全基因序列仅有1988bp，与中国台湾代表株属于同一进化分支，属于基因2型；Cap蛋白和Rep蛋白的氨基酸序列的变异分析表明Cap蛋白的变异程度要显著高于Rep蛋白。

Abstract:

In order to understand the genetic characteristics and variation of the duck circovirus strains in recent years, the comp genome amplification, cloning, sequence analysis and genetic evolution analysis of 30 duck circovirus positive samples in some regions of China were carried out by PCR . The results showed that the full gene sequence homology between the 30 strains of DuCV strains were 85.8%~99.9%, and the full-length sequence of 26 DuCV were 1993~1995 bp, which were in the same evolutionary branch with the German representative strain. They belong to gene type 1, and the full-length sequence of 4 DuCV were only 1988 bp, which belong to the same evolutionary branch as the representative strain of Taiwan, and belong to gene type 2; the variation analysis of the amino acid sequences of Cap protein and Rep protein indicated that the degree of variation of Cap protein was significant. Higher than Rep protein.