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牛奶中氟喹诺酮类药物残留同步快速检测的酶联免疫吸附法研究
李燕君,谭梅,岳秀英,陆强,吴晓岚,吕晓华
0
(四川大学华西公共卫生学院/四川大学华西第四医院)
摘要:
建立同时快速检测牛奶中环丙沙星、恩诺沙星、诺氟沙星、培氟沙星、氧氟沙星和洛美沙星六种氟喹诺酮类药物残留的酶联免疫吸附法。ELISA法的操作在20℃~25℃下进行,以50%抑制浓度、最低检测限反映方法的灵敏度,同时进行加标回收试验。结果表明,本文所建立的ELISA法的最低检测限为1.48μg/L,50%抑制浓度的范围在0.254~0.361μg/L;在环丙沙星、恩诺沙星、诺氟沙星、培氟沙星、氧氟沙星和洛美沙星药物添加浓度为5μg/L、25μg/L、50μg/L时的回收率为84.58%~115.92%,批内变异系数均小于15.0%,批间变异系数均小于16.0%。本方法简便快捷,灵敏度、准确度和精密度高,适用于牛奶中FQs残留的同时快速检测。
关键词:  酶联免疫吸附法  牛奶  氟喹诺酮类药物  残留
DOI:
投稿时间:2019-09-03修订日期:2019-12-24
基金项目:四川省科技计划(2019YFS00620),四川大学高校基本科研业务费研究专项项目(skqy201234)
Determination of Fluoroquinolones Residues in Milk by Enzyme-linked Immunosorbent Assay
(West China School of Public Health and West China Fourth Hospital,Sichuan University)
Abstract:
An enzyme-linked immunosorbent assay was established for the simultaneous determination of six fluoroquinolone residues in milk, includes ciprofloxacin, enoxacin, norfloxacin, pefloxacin, ofloxacin and lomefloxacin. ELISA was performed at 20℃~25℃, the sensitivity of the method was reflected by 50% inhibition concentration and minimum detection limit, the recovery test was carried out at the same time. The results showed that the minimum detection limit of this method was 1.48 μg/L, the range of 50% inhibitory concentration was 0.254~0.361μg/L; The recovery rate of ciprofloxacin, enoxacin, norfloxacin, pefloxacin, ofloxacin and lomefloxacin at 5μg/L, 25μg/L and 50μg/L was 84.58%~115.92%, the intra-batch variation coefficient was less than 15.0%, and the inter-batch variation coefficient was less than 16.0%. This method is simple and rapid, with high sensitivity, accuracy and precision. It is suitable for the rapid screening and detection of the residues of fluoroquinolones in milk.
Key words:  ELISA  milk  fluoroquinolones  residues

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