响应面法优化超声波提取浮萍多糖工艺研究

The Response Surface Method to Optimize the Ultrasonic Extraction Duckweed Polysaccharide Technology was Studied

关键词:  浮萍多糖  超声波处理  单因素试验  响应面分析

Key words:  Spirodela polyrrhiza (L.)Schleid Polysaccharide  Ultrasonic Treatment  Single Factor Experiment  Response Surface Methodology

摘要:

为优化浮萍多糖的提取工艺，基于单因素试验结果对影响浮萍多糖提取率的三个显著因素及水平：料液比(1∶40、1∶50、1∶60)、超声时间(40 min、60 min、80 min)和超声功率(300 W、350 W、400 W)进行了研究。以浮萍多糖提取率为响应值，运用Design-Expert软件进行响应面设计并开展试验，通过工艺优化确定浮萍多糖的最佳提取条件为料液比1∶40.06、超声时间79.58 min、超声功率341.37 W，此条件下浮萍多糖提取率的理论值可达到1.188%；验证试验的浮萍多糖提取率为1.125%，与理论值仅相差0.063%。优化后的提取工艺条件准确可靠，可用于实际操作。

Abstract:

In order to optimize the extraction process of duckweed polysaccharides, three significant factors and levels affecting the extraction rate of duckweed polysaccharides were studied based on single factor test results, including the ratio of material to liquid (1∶40, 1∶50, 1∶60)，ultrasonic time (40 min, 60 min, 80 min)， and ultrasonic power (300 W, 350 W, 400 W). The response rate of the extract of duckweed was taken as the response value, and the design of the experimental scheme was optimized by a Design-Expert software. The optimal extraction conditions of duckweed polysaccharides were determined by process optimization: the ratio of material to liquid was 1∶40.06, the ultrasonic time was 79.58 min, and the ultrasonic power was 341.37 W. Under this condition, the theoretical extraction value of duckweed polysaccharides could reach 1.188%. It was verified by experiments that the extraction rate of duckweed polysaccharide was 1.125%, which was only 0.063% different from the theoretical value. The optimized extraction process conditions are accurate and reliable and can be used for practical operations.