响应面法优化琼辣蓼总黄酮提取工艺及其抗氧化活性研究

Optimization of Extraction Process of Total Flavonoids from Polygonum hydropiper by Response Surface Methodology and Its Antioxidant Activity

关键词:  琼辣蓼  总黄酮  提取工艺  抗氧化  响应面法

Key words:  Polygonum hydropiper  total flavonoids  extraction process  antioxidant activity  response surface methodology

摘要:

为优化海南辣蓼总黄酮的提取工艺条件并探讨总黄酮体外抗氧化活性，采用热回流提取海南辣蓼总黄酮，以提取液中总黄酮的百分含量为考察指标，在单因素试验基础上，运用Box-Behnken 设计(BBD) 对影响提取工艺的因素液料比、提取时间及乙醇体积分数进行分析，应用响应面法优选最佳提取工艺；采用清除1，1-二苯基-2-三硝基苯肼(DPPH) 自由基和羟基自由基的方法，对最佳工艺所得总黄酮进行抗氧化活性评价。结果表明：海南辣蓼总黄酮的最佳提取工艺条件为：液料比1∶15、提取时间60 min、乙醇体积分数48%，在此条件下总黄酮提取率为10.101%。总黄酮对羟基自由基和DPPH自由基的半数抑制浓度(IC50)分别为2.95 mg/mL和0.46 mg/mL。该方法优选的工艺合理，操作简便，试验周期短，且提取的海南辣蓼总黄酮具有良好的抗氧化活性，为琼辣蓼的深入开发利用提供了实验依据。

Abstract:

To optimize the extract condition of total flavonoids in Polygonum hydropiper by response surface methodology and study its antioxidant activity in vitro,the total flavonoids of Polygonum hydropiper was extracted by hot reflux method. Taking the percentage content of total flavonoids as the evaluation index,The factors: the solid-liquid ratio, extraction time and ethanol concentration that affect extraction process were analyzed by Box-Behnken design on the basis of single factor experiment, and response surface method was used to optimize extraction technology. Antioxidant activity of total flavonoids in vitro was valuated by DPPH and hydroxyl free radical scavenging activity assay. The optimum extraction conditions was as follows: solid-liquid ratio 1:15, extraction time 60 min, and under the optimal condition, the extraction rate of total flavonoids was 10.101%. The experiments showed that the half inhibitory concentration(IC50) of hydroxyl radical and DPPH radical was 2.95 mg/mL, 0.46 mg/mL respectively. The optimal extraction technology was verified as reasonable, feasibleand short test cycle, and total flavonoids have good antioxidant effects in vitro, which provides experimental basis for the further development and utilization of Polygonum hydropiper.