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HPLC法测定盐酸左旋咪唑片的含量研究
周芷锦
0
(浙江省兽药饲料监察所)
摘要:
建立盐酸左旋咪唑片中盐酸左旋咪唑含量测定的高效液相色谱法。采用Agilent Eclipse XDB-C18(250 mm×4.6 mm),5 μm色谱柱,Agilent 1260DAD检测器,流动相为0.05 mol/L磷酸二氢钾溶液(三乙胺调节pH值至7.0)-乙腈(80∶20,v/v);进样量为20 μL;柱温为30 ℃;流速为1.0 mL/min;检测波长为214nm。结果表明:盐酸左旋咪唑在1.024~102.398 μg/mL范围内线性关系良好(r=1.0000),高、中、低浓度回收率分别为97.4%、99.7%、99.3%。该方法定性、定量准确,适用于盐酸左旋咪唑片中盐酸左旋咪唑的含量测定。
关键词:  盐酸左旋咪唑片  高效液相色谱法  二极管阵列检测器
DOI:
投稿时间:2019-06-04修订日期:2019-07-24
基金项目:
Determination of Levamisole Hydrochloride in Levamisole Hydrochloride Tablets by HPLC
(Zhejiang Province Supervisory Institute of Veterinary Drug and Feed)
Abstract:
To establish a method for the determination of Levamisole Hydrochloride in Levamisole Hydrochloride Tablets by HPLC. Agilent Eclipse XDB-C18 (250 mm×4.6 mm),5μm column was used. The mobile phase was potassium dihydrogen phosphatephosphoric (0.05 mol/L,pH value adjusted to 7.0 with triethylamine)-acetonitrile (80∶20, v:v), the injection volume was 20 μL, the column temperature was 30 ℃, the flow rate was 1.0 mL/min and detected at the wavelength of 214 nm.The result indicated that a good linear relationship was accepted in the range of 1.024~102.398 μg/mL(r=1.0000).and the recovery of high,medium,low-Concentration were 97.4%,99.7%,99.3%.The method is accurate in qualitative and quantitative analysis, and suitable for the determination of Levamisole Hydrochloride Tablets.
Key words:  Levamisole Hydrochloride Tablets  High performance liquid chromatography  Diode array detector

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