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水貂犬瘟热Vero细胞活疫苗(CDV3-CL株,悬浮培养)安全性评价
冯二凯,易立,罗国良,王振军,郭利,陈立志,程世鹏,程悦宁
0
(中国农业科学院特产研究所;吉林特研生物技术有限责任公司)
摘要:
为考察新研制的水貂犬瘟热Vero细胞活疫苗(CDV3-CL株,悬浮培养)的安全性,分别以一次单剂量(1头份)、单剂量重复和一次超剂量(10头份)接种动物,对疫苗进行全方位安全性评价,试验动物涉及水貂幼崽、育成水貂以及妊娠水貂等,重点观察注射动物的体温、食欲,生产性能是否发生改变、注射部位是否出现炎症反应以及实验动物接种部位组织病变等,连续观察15 d,结果发现,实验动物没有发生炎症反应,动物体温变化也处于正常范围内,证明疫苗是安全的,从而为疫苗的推广奠定基础。
关键词:  水貂  犬瘟热疫苗  悬浮培养  安全性评价
DOI:
投稿时间:2019-05-31修订日期:2019-07-21
基金项目:吉林省科技发展计划(20150204073NY);吉林省“双十”重大科技攻关(20150201006NY)
Safety Evaluation of Newly Prepared Mink Attenuated Canine Distemper Live Vaccine (CDV3-CL Strain, Suspension)
(Institution of Special Animal and Plant Science of Chinese Academic of Agriculture Science,Jilin Changchun;Jilin Teyan Biotechnology Co,Ltd,Jilin Changchun)
Abstract:
In order to evaluate the security of the newly prepared mink attenuated live vaccine (CDV3-CL strain,suspension), the minks (mink cubs, aged mink or pregnant mink), whose the serum-neutralizing antibody is under 1∶4, were random grouped and inoculated with different dose of vaccine, include one dose, repeated one dose and overdose inculated. The nonspecific safety evaluation showed that all the experimental animals living healthy, the temperature fluctuation are under the control and there had no die or inflammation happened. In addition, the histopathological section result indicated that all the muscle around the inject site is as norm as the control. The above results demonstrated that the mink attenuated live vaccine(CDV3-CL strain, suspension)was secure and can be used on a large scale in fields.
Key words:  mink  canine distemper live vaccine  suspension  security evaluation

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