广西某健康鸡群Ⅰ群禽腺病毒的hexon蛋白loop1基因遗传进化分析

Genetic Evolution Analysis of Loop 1 Gene of hexon Protein Fowl Adenovirus Group Ⅰ in Healthy Chicken in Guangxi

关键词:  Ⅰ 群禽腺病毒  loop1基因  遗传进

Key words:  FowlSadenovirusSgroupSⅠ  hexon gene  genetic evolution

摘要:

为调查广西某规模化鸡场健康鸡群Ⅰ群禽腺病毒(FAdV-Ⅰ)的感染情况，于2017年11月-2018年12月采集不同日龄鸡群咽喉拭子和泄殖腔拭子样品4700份，对样品进行FAdV-Ⅰ检测和hexon蛋白loop 1基因扩增，应用LaserGene7.1和MEGA4.1软件对 loop 1基因序列进行核苷酸和遗传进化分析。结果显示，FAdV-Ⅰ的阳性检出率为8.72%；40~70日龄和71~99日龄鸡群阳性FAdV-Ⅰ的检出率分别为28.17%和22.91%；测序获得的38条 loop 1基因序列分析表明该鸡场健康鸡群感染了5个不同种的FAdV-Ⅰ，其中26.32%的序列为A种(FAdV-A)、2.63%为B种(FAdV-B)、10.53%为C种(FAdV-C)、39.47%为D种(FAdV-D)和21.05%为E种(FAdV-E)；遗传进化分析表明该鸡场健康鸡群主要感染的种为FAdV-D，血清2型(FAdV-2)为优势血清型，其次为A种的血清1型(FAdV-1)和E种的血清8a型(FAdV-8a)和8b型(FAdV-8b)。结果表明，40~70日龄健康鸡群的FAdV-Ⅰ的检出率最高，在健康鸡群中感染的FAdV-Ⅰ主要为FAdV-D种中的FAdV-2。

Abstract:

To investigate the infection of fowl adenovirus group Ⅰ (FAdV-Ⅰ) in healthy chicken flocks of a large-scale chicken farm in Guangxi, 4700 samples of throat swabs and cloacal swabs of different ages chickens were collected from November 2017 to December 2018; FAdV-Ⅰdetection and loop1 genes of hexon protein amplification were carried out. LaserGene7.1 and MEGA4.1 software were used to analyze the nucleotide and genetic evolution of loop1 genes. The results showed that the positive detection rate of FAdV-Ⅰwas 8.72%; the detection rate of FAdV-Ⅰ in aged 40~70 days and aged 71~99 days were 28.17% and 22.91%, respectively. Genetic analysis of the 38 loop1 genes obtained by sequencing showed that healthy chickens of this farm were infected with different five species of FAdV-Ⅰ, of which 26.32% were species group A (FAdV-A), 2.63% were species group B (FAdV-B), 10.53% were species group C (FAdV-C), 39.47% were species group D (FAdV-D), and 21.05% were species group E (FAdV-E), respectively. Genetic evolution analysis showed that the dominant species group was FAdV-D, serotype 2, followed by serotype 1 (FAdV-1) of species group A and serotype 8a and 8b (FAdV-8a and FAdV-8b) of species group E. The results showed that the detection rate of FAdV-Ⅰ in healthy chickens aged 40~70 days was the highest, and FAdV-2 of FAdV-D was dominant serotype in healthy chickens infected with FAdV-Ⅰ.