UPLC法同时测定桃金娘果中4种酚类成分

Simultaneous determination of four main phenolics in Rhodomyrtustomentosa(Ait.) Hassk. fruit by UPLC

关键词:  超高效液相色谱  桃金娘果  没食子酸  鞣花酸  白皮杉醇  白藜芦醇

Key words:  ultra performance liquid chromatography  Rhodomyrtustomentosa(Ait.) Hassk.fruit  gallic acid  ellagic acid  piceatannol  resveratrol

摘要:

建立了UPLC同时测定桃金娘果中没食子酸、鞣花酸、白皮杉醇和白藜芦醇4种酚类物质的方法。桃金娘果样品经甲醇超声提取后采用Waters CORTECS?UPLC?T3色谱柱（2.1 mm×100 mm，1.6 μm）；流动相为乙腈-0.2%磷酸溶液进行梯度洗脱程序；流速0.2 mL/min；柱温35℃；检测波长为280 nm。结果显示桃金娘果中的4种成分10 min内能够完全分离，在测定的质量浓度范围内线性关系良好，相关系数分均大于0.9990；平均加样回收率在95.1%～102%之间，RSD 1.5%～2.8%；检出限分别为7.55 ng/mL、2.32 ng/mL、63.7 ng/mL、4.59 ng/mL。该法简便快速，具有较高准确性和重复性，可用于桃金娘果药材的质量控制。

Abstract:

An ultra performance liquid chromatography method for the determination offour phenolicsin Rhodomyrtustomentosa(Ait.) Hassk. fruitsimultaneously (gallic acid, ellagic acid, piceatannol and resveratrol) was obtained. The samples were extracted by methanol and detected through a Waters CORTECS?UPLC?T3 column (2.1×100 mm 1.6 μm) with the mobile phaseof acetonitrile-0.2% phosphoric acid,following a flow rate of 0.20 mL/min.The detection wave-length was set at 280 nm and thecolumn temperature was 30 ℃.The results showed that four compounds can be completely separated within 10 min. The calibration curves for the four compounds were linear within a specific range respectively with a related coefficient more than 0.9990. Their respective average recovery were in the range of 95.1%～101.9% and the RSD in the range of 1.5%～2.8%.The limit detection of gallic acid, ellagic acid, piceatannol and resveratrol were 7.55, 2.32, 63.7, 4.59 ng/mL, respectively. This simple and rapid method can be used for the quality control of Rhodomyrtustomentosa(Ait.) Hassk.fruitwith high accuracy and repeatability.