穿心莲超声提取工艺研究

Study on Ultrasonic Extraction Process of Andrographis paniculata

关键词:  穿心莲  二萜内酯类  超声提取  提取工艺  单因素  正交试验

Key words:  Andrographis paniculata  Diterpene lactones  Ultrasonic extraction  Extraction process  Single factor  Orthogonal test

摘要:

优选穿心莲药材的提取工艺。通过单因素和正交试验,测定穿心莲内酯、脱水穿心莲内酯、新穿心莲内酯三种二萜内酯类的总含量,确定最佳超声提取工艺。确定穿心莲药材的最佳超声提取工艺为乙醇浓度60%,超声时间1小时,料液比为1:15,此条件下穿心莲二萜内酯类的总含量为2.27%。本实验确定的超声提取工艺稳定可行,质量可靠,可为穿心莲有效成分的提取提供理论依据。

Abstract:

Optimization of Extraction Process of Andrographis paniculata.Through single factor and orthogonal tests, the total contents of andrographolide, dehydroandrographolide and Neoandrographolide were determined to determine the optimal ultrasonic extraction process. The optimal ultrasonic extraction process of Andrographis paniculata was determined as follows: ethanol concentration 60%, ultrasonic time 1 hour, solid-liquid ratio 1:15. Under these conditions, the total content of Andrographis paniculata diterpenoids was 2.27%. The ultrasonic extraction process determined by this experiment is stable and feasible, with reliable quality, which can provide theoretical basis for the extraction of effective components of Andrographis paniculata.