引用本文
  •    [点击复制]
  •    [点击复制]
【打印本页】 【下载PDF全文】 查看/发表评论下载PDF阅读器关闭

←前一篇|后一篇→

过刊浏览    高级检索

本文已被:浏览 99次   下载 95 本文二维码信息
码上扫一扫!
高效液相色谱法同时测定博落回注射液中血根碱和白屈菜红碱的含量
赵有轩,张传津,张志民,章安源
0
(山东省兽药质量检验所)
摘要:
建立了高效液相色谱法测定博落回注射液中血根碱和白屈菜红碱含量的方法。采用Agilent1260型高效液相色谱仪,配二极管阵列检测器(PDA),色谱柱为waters Symmetry C18(4.6×250mm,5μm),柱温:35℃,流速为:1.0ml/min,流动相:乙腈-三乙胺溶液=25:75(磷酸调pH值至2.5),检测波长270nm。结果表明:血根碱在0.5~100μg/ml范围内线性关系良好(R2=1.000),平均回收率(n=6)为98.21%,RSD为1.76%;白屈菜红碱在0.25~50μg/ml范围内线性关系良好(R2=1.000),平均回收率(n=5)为98.77%,RSD为0.83%。本方法简便快捷,可用于博落回注射液中血根碱和白屈菜红碱的含量测定。
关键词:  高效液相色谱法  博落回注射液  血根碱  白屈菜红碱
DOI:
投稿时间:2019-04-23修订日期:2019-06-21
基金项目:
Simultaneous Determination of Sanguinarine and Chelerythrine in Macleaya Cordata Injection by HPLC-PDA
(Institute of veterinary drug quality inspection of Shandong Province)
Abstract:
This study was amid to establish a high performance liquid chromatography –diode array (HPLC-PDA) test method for determination of Sanguinarine and Chelerythrine in macleaya cordata injection. Chromatographic conditions are as follows: separate column is Waters Symmetry C18 (4.6×250 mm, 5 μm), the column temperature is 35℃, the flow rate is 1.0 ml/min, mobile phase consists of acetonitrile and triethylamine, volume ration is 25:75,adjust pH to 2.5 with phosphoric acid, and the detection wavelength is 270 nm. The results show that, the linearity of the method for sanguinarine detection complies with the requirement at the range of 0.5-100 μg/ml (R2=1.000), the average recovery rate is 98.21%, RSD=1.76%. The linearity of the method for chelerythrine detection complies with the requirement at the range of 0.25-50 μg/ml (R2=1.000), the average recovery rate is 98.77%, RSD=0.83%. This method is simple, quick, and it can be used for determination of sanguinarine and chelerythrine in macleaya cordata injection.
Key words:  HPLC  macleaya cordata injection  sanguinarine  chelerythrine

用微信扫一扫

用微信扫一扫