板蓝根指标成分分布分析及药材质量评价研究

Distribution Analysis of Index Components in Radix Isatidis and Research of its medicinal materials quality evaluation

关键词:  板蓝根  核苷  靛蓝  靛玉红  产地分析  质量评价

Key words:  Radix Isatidis  nucleoside  indigo  indirubin  origin analysis  quality evaluation

摘要:

为测定多产地板蓝根药材中精氨酸、靛蓝、靛玉红、（R，S）-告依春、核苷类含量，分析含量分布情况，并进行板蓝根药材质量评价研究。采用高效液相色谱法（HPLC）测定不同产地来源的11个板蓝根样品7个指标成分含量，通过因子分析和分层聚类分析方法进行成分分布分析和质量评价研究。结果表明靛蓝、靛玉红、鸟苷、尿苷、腺苷对不同产地板蓝根评价贡献最大，这5种指标成分可作为产区间区分和评价指标，以此评价体系板蓝根按产区分为河北省产区、甘肃-山东-安徽产区和东北三省产区，其中河北省产板蓝根品质最优异。

Abstract:

In order to determine the content of arginine, indigo, indirubin, epigoitri and nucleosides, analyze the content distribution and study the quality evaluation of Radix Isatidis, the contents of 7 indicators of 11 samples were determined by High Performance Liquid Chromatography (HPLC) and the composition analysis and quality evaluation were carried out by factor analysis and systematic cluster analysis. Factor analysis showed that the evaluation of indigo, indirubiIn order to determine the content of arginine, indigo, indirubin, epigoitri and nucleosides, analyze the content distribution and study the quality evaluation of Radix Isatidis, the contents of 7 indicators of 11 samples were determined by High Performance Liquid Chromatography (HPLC) and the composition analysis and quality evaluation were carried out by factor analysis and systematic cluster analysis. Factor analysis showed that the evaluation of indigo, indirubin and nucleosides contributed the most to the Radix Isatidis from different habitats. Cluster analysis can classify Radix Isatidis according to the production area by using the five indicators selected in factor analysis. The three production areas were Hebei Province, Gansu-Shandong-Anhui production area and northeastern three provinces. The factor score of the Radix Isatidis of Hebei Province were the highest, evaluated by the comprehensive factor scores. The results showed that the indigo, indirubin and nucleoside components of Radix Isatidis can be used as an indicator for the differentiation and evaluation of the production interval, for it is affected by the difference in the production area. The quality of the Radix in Hebei province was the best according to the established evaluation system, and the production of Radix was divided into Hebei Province area, Gansu-Shandong-Anhui area and the three provinces in Northeast China area.n and nucleosides contributed the most to the Radix Isatidis from different habitats. Cluster analysis can classify Radix Isatidis according to the production area by using the five indicators selected in factor analysis. The three production areas were Hebei Province, Gansu-Shandong-Anhui production area and northeastern three provinces. The factor score of the Radix Isatidis of Hebei Province were the highest, evaluated by the comprehensive factor scores. The results showed that the content of Arginine and epigoitri was not closely related to the production area. The indigo, indirubin and nucleoside components of Radix Isatidis can be used as an indicator for the differentiation and evaluation of the production interval, for it is affected by the difference in the production area. The quality of the Radix in Hebei province was the best according to the established evaluation system.