2017-2018年吉林部分地区规模化猪场猪细环病毒的遗传变异分析

Analysis of TTSuVs prevalence and genetic variation of pig in the some region of Jilin from 2017 -2018

关键词:  TTSuVs  流行情况  ORF1  遗传变异

Key words:  TTSuVs  epidemic  ORF1  genetic variation

摘要:

为了解猪细环病毒（Torque teno sus virus, TTSuVs）在吉林部分规模化猪场的流行及其遗传变异情况。本研究对2017-2018年间，来自吉林地区10家规模化猪场的574份血清样本进行TTSuVs流行情况调查。并根据收地点，对20株TTSuVs进行ORF1全基因序列扩增及遗传变异性分析。结果显示：TTSuV1感染率为35.0%；TTSuV2感染率为63.2%；TTSuV1与TTSuV2混合感染率为30.3％；10株TTSuV1测序株之间的核苷酸同源性为75.7%-94.7%，氨基酸同源性为48.1%-88.2%；10株TTSuV2测序株之间的核苷酸同源性为77.5%-93.7%，氨基酸同源性为70.6%-87.4%；且吉林地区流行的TTSuV1均属于TTSuV1-lb型，而TTSuV2流行情况较为复杂，各种亚型均有涉及。

Abstract:

In order to clarify the prevalence and genetic variation of Torque teno sus virus (TTSuVs) in Jilin. The 574 serum samples from 10 large-scale pig farms in Jilin investigated. According to the location of the sample collection, 20 TTSuVs were amplified by ORF1 gene sequence and genetic variation analysis. The results showed that the infection rate of TTSuV1 was 35.0%; the infection rate of TTSuV2 was 63.2%; The mixed infection rate of TTSuV1 and TTSuV2 was 30.3%; The nucleotide homology between 10 TTSuV1 sequencing strains was 75.7%-94.7%, and the amino acid was 48.1%-88.2%; The nucleotide homology between 10 TTSuV2 sequencing strains was 77.5%-93.7%, the amino acid homology was 70.6%-87.4%; The popular TTSuV1 in Jilin belongs to TTSuV1-lb , while the prevalence of TTSuV2 was more complicated, and various subtypes were involved..