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超高效液相色谱-紫外检测法测定肾复康合剂中落新妇苷和芦丁的含量
魏秀丽,丁新仁,徐恩民,栾明娜,李有志,孙亚磊
0
(济南市槐村街68号;山东齐发药业有限公司)
摘要:
本研究建立超高效液相色谱(UPLC)测定肾复康合剂中主成分落新妇苷和芦丁的含量。采用反相高效液相色谱法对落新妇苷和芦丁进行色谱分离和快速定量测定。以ACQUITY UPLCTM C18色谱柱(2.1mm×100mm,1.7μm)为分离柱,柱温:35℃;以乙腈-0.1%甲酸水溶液,进行梯度洗脱;流速:0.35mL/min;进样量:5μL;检测波长为204nm。通过光谱图、保留时间和峰面积参数对落新妇苷和芦丁进行定性定量检测。落新妇苷在2~75μg/mL的范围内线性关系良好(R2>0.998);方法检出限为1μg/mL,方法定量限为2μg/mL; 芦丁在4~150μg/mL的范围内线性良好(R2>0.996);方法检出限为2μg/mL,方法定量限为4μg/mL,完全满足检测需求。该方法色谱分离较好,分析速度较快,前处理简单,适用于肾复康合剂中落新妇苷和芦丁的定性定量检测。
关键词:  肾复康合剂  超高效液相色谱-二极管阵列检测法  落新妇苷  芦丁
DOI:
投稿时间:2019-03-31修订日期:2019-06-03
基金项目:2018年山东省重点研发计划“家禽健康养殖天然药物的研制与开发”编号2018GNC111010。
Determination of Astilbin and Rutin in shenfukang mixture by UPLC-UV
(Shandong Provincial Veterinary Medicine Supervision Institute,Jinan)
Abstract:
This study was aimed to establish a high performance liquid chromatography (UPLC-UV) test method for the determination of Astilbin and Rutin in shenfukang mixture.UPLC was used to isolate and quantitatively determine of Astilbin and Rutin. ACQUITY UPLCTM C18 chromatographic column (2.1mm x 100mm,1.7 mm) was used as the separation column, Column temperature 35℃; Gradient elution was performed with acetonitrile -0.1% formic acid solution; The flow rate is 0. 35mL/ min, the injection volume is 5 μ L, and the detection wavelength is 204nm. In this paper, the quantitative and qualitative analysis of Astilbin and Rutin was carried out by spectrogram,retention time and peak area parameters. In the range of 2~75μg/mL, the linear relationship of Astilbin is good (R2>0.998); the detection limit of the method is 1μg/mL, and the quantitative limit of the method is 2μg/mL; In the range of 4~150μg/mL, the linear relationship of Rutin is good (R2>0.996); The detection limit of the method is 2μg/mL(S/N>3), and the quantitative limit of the method is 4μg/mL(S/N> 10), fully meet testing requirements. The method has good separation performance, fast analysis speed and simple pretreatment, suitable for the qualitative and quantitative detection of Astilbin and Rutin in shenfukang mixture.
Key words:  Shenfukang mixture  High performance liquid chromatography-diode array detection method  Astilbin  Rutin

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