增精宝对环磷酰胺致小鼠生精障碍保护作用的研究

Protective Effect of ZengJingBao on Spermatogenesis Disturbance Induced by Cyclophosphamide in Mice

关键词:  增精宝  环磷酰胺  小鼠  生精作用

Key words:  ZengJingBao  cyclophosphamide  mice  spermatogenesis

摘要:

为评价增精宝对雄性动物生精作用的影响，采用环磷酰胺致小鼠生精障碍为动物模型，考察不同剂量增精宝对环磷酰胺致小鼠生精障碍的保护作用。结果显示，高剂量组对小鼠的附睾、睾丸、储精囊和前列腺均有较显著的促进作用；低剂量组精子总密度和精子活率均差异显著（P＜0.05）；高剂量组精子活率差异显著（P＜0.05）；对小鼠睾丸各倍体生精细胞比例的影响，高剂量组小鼠睾丸生精小管较为饱满，管腔内存在大量精子，生精上皮较厚，可见各级生精细胞排列整齐，界膜明显，睾丸间质细胞排列整齐。结论：增精宝两种剂量均能够显著的促进环磷酰胺导致小鼠生精障碍的保护作用，高剂量组对雄性小鼠的作用尤为显著。

Abstract:

In order to evaluate the effect of ZengJingBao on spermatogenesis in male animals, cyclophosphamide-induced spermatogenesis disorder in mice was used as an animal model to investigate the protective effect of different doses of ZengJingBao on spermatogenesis disorder in mice induced by cyclophosphamide. The results showed that the high-dose group had significant promoting effects on the epididymis, testis, seminal vesicle and prostate of mice; the low-dose group had significant differences in total sperm density and sperm viability (P＜0.05); the high-dose group had significant differences in sperm viability (P＜0.05); Effects of the proportion of spermatogenic cells in each ploid in the high-dose group showed that the seminiferous tubules were full, and there were plenty of sperm in the lumen, the seminiferous epithelium was thicker. It could be seen that the spermatogenic cells at all levels were arranged neatly, the boundary membrane was obvious, and the Leydig cells of the testis were arranged neatly. CONCLUSION: Both doses of ZengjingBao can significantly promote the protective effect of cyclophosphamide on spermatogenesis disorder in mice, especially, high-dose of ZengjingBao was effective in male mice.