鱼腥草芩蓝口服液质量控制方法研究

Study on Quality Control Method of Houttuynia cordata Bailan Oral Liquid

关键词:  鱼腥草芩蓝口服液  薄层鉴别  含量测定

Key words:  Yuxingcao Qinlan oral liquid  TLC  determination of content

摘要:

为建立鱼腥草芩蓝口服液的质量控制方法，采用TLC法对鱼腥草芩蓝口服液中的鱼腥草、黄芩、连翘和金银花进行鉴别；采用HPLC法测定黄芩苷和绿原酸的含量。在薄层色谱鉴别中均能检出鱼腥草、黄芩、连翘和金银花的特征性斑点；黄芩苷进样量在0.1057～2.6413 μg范围内与峰面积有良好的线性关系，回归方程为：Y=33.42325X-214.6128，r=0.9997，平均回收率为98.74%；绿原酸进样量在0.041～2.051 μg/mL范围内与峰面积有良好的线性关系，其回归方程为：Y=-86.78088+27.5937X，相关系数r=0.9995，回归显著，平均回收率为99.1%，建立的黄芩苷和绿原酸含量测定方法，耐受性良好、灵敏度高、重现性好、专属性强，可以有效控制产品的质量。建立的方法能准确、可靠地对鱼腥草芩蓝口服液中黄芩苷和绿原酸含量进行测定，可作为该制剂的质量控制方法。

Abstract:

To establish a quality control method for Yuxingcao Qinlan oral liquid. Houttuynia cordata, Astragalus membranaceus, Forsythia suspensa and Honeysuckle in Yuxingcao Qinlan oral Liquid were identified by TLC method. The content of baicalin and chlorogenic acid was determined by HPLC.The characteristic spots of Houttuynia cordata, Astragalus membranaceus, Forsythia suspensa and Honeysuckle can be detected in the thin layer chromatography. The amount of baicalin injection in the range of 0.1057～2.6413 μg has a good linear relationship with the peak area. The regression equation is: Y=33.42325X-214.6128, r=0.9997, the average recovery was 98.74%; the chlorogenic acid injection amount in the range of 0.041～2.051 μg/mL had a good linear relationship with the peak area, and the regression equation was:Y=-86.78088+27.5937X,correlation coefficient r=0.9995, significant regression, average recovery rate is 99.1%, the determination method of baicalin content and chlorogenic acid content established in the standard, good tolerance, high sensitivity, reproducibility Good, specific, can effectively control the quality of the product. The method could accurately and reliably determine the content of baicalin and chlorogenic acid in Yuxingcao Qinlan oral Liquid, and could be used as a quality control method for the preparation.