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高效液相色谱法测定清肺止咳散中黄芩苷含量
朱育红,朱弘
0
(陕西省兽药监测所)
摘要:
为了测定清肺止咳散中黄芩苷的含量,采用C18柱(5 μm,250 mm×4.6 mm),在室温下,以甲醇-0.3%磷酸溶液(55:45)为流动相,以276 nm为检测波长,流速为1.0 ml/ min,柱温30℃,建立了高效液相色谱测定方法。黄芩苷在3.8~379 μg/ml范围内线性良好, R2 =0.9998,平均回收率为94.3%(n=6),RSD为0.92%。本方法简便、准确、可靠,可用于控制清肺止咳散中黄芩苷的含量。
关键词:  高效液相色谱法  清肺止咳散  黄芩苷
DOI:
投稿时间:2019-01-02修订日期:2019-05-30
基金项目:
The content of baicalin in Qingfei Zhike Powder was determined by HPLC
(Shaanxi Provincial Veterinary Drug Monitoring Institute)
Abstract:
To determine the content of Baicalin in Qingfei Zhike Powder. Using C18 column(5 μm 250mm×4.6mm),At room temperature, methanol-0.3% phosphoric acid solution (55:45) was used as the mobile phase, The detection wavelength was 276 nm, The flow rate of 1.0 ml/min,The temperature of column is 30℃.The results showed, Baicalin had a good linear relationship in 3.8~379.0 in the range of igg /ml, R2 =0.9998, The average recovery rate was 94.3% and RSD 0.92%. This method is convenient accurate and fast, and can be used to control the content of scutellaria baicalensis in Qingfei Zhike Powder.
Key words:  HPLC  Qingfei Zhike Powder  Baicalin

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