伊维菌素吡喹酮咀嚼片含量测定方法的建立

Establishment of the Content Determination Method for Ivermectin and Praziquantel Chewable Tablets

关键词:  伊维菌素吡喹酮咀嚼片  含量测定  伊维菌素  吡喹酮

Key words:  Ivermectin and praziquantel chewable tablets  Content determination  Ivermectin  Praziquantel

摘要:

为了建立伊维菌素吡喹酮咀嚼片同时测定两种主药含量的方法，采用十八烷基硅烷键合硅胶为填充剂，以乙腈：水的梯度洗脱系统作为流动相，伊维菌素检测波长为245nm，吡喹酮检测波长为210nm。在该色谱条件下，溶剂和辅料对伊维菌素和吡喹酮出峰均无干扰，伊维菌素和吡喹酮与相邻杂质峰分离良好。伊维菌素在2~400μg/ml的浓度范围线性关系良好；吡喹酮在1~100μg/ml的浓度范围内线性关系良好。伊维菌素回收率为102.5%，吡喹酮回收率为99.4%。该方法操作简便，准确度高，重复性好，可用于伊维菌素吡喹酮咀嚼片同时测定两种主药的含量。

Abstract:

In order to establish a content determination method for simultaneous determinating two main drugs in ivermectin and praziquantel chewable tablets, octadecyl silane bonded silica gel was used as filler, acetonitrile-water gradient elution system was used as mobile phase, the detection wavelength of ivermectin was 245 nm and the detection wavelength of praziquantel was 210nm. In this method, solvents and excipients did not interfere with the peaking of ivermectin and praziquantel, ivermectin and praziquantel were well separated from adjacent impurity peaks. The linear relationship of ivermectin in the concentration range of 2-400 μg/ml was good. The linear relationship of praziquantel in the concentration range of 1-100 μg/ml was good. The recovery of ivermectin was 102.5%. The recovery of praziquantel was 99.4%. The method is simple, accurate and reproducible, which can be used for the simultaneous determination of two main drugs in ivermectin and praziquantel chewable tablets.