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黄芪多糖注射液生物活性检测方法的分析与评价
元雪浈,马发顺,段沙沙,王军红,王小艳,李华岑,刘占通,周红霞,吴志明
0
(河南省兽药饲料监察所)
摘要:
为了证实现有黄芪多糖生物活性检测方法的效果,并确定胸腺重和胸腺指数在黄芪多糖生物活性检测中的作用,使用《中国兽药典2015版(二部)》提供的检测方法,对河南省4个兽药厂提供的黄芪多糖样品进行了生物活性检测,测量了昆明鼠的体重、脾脏重和胸腺重等,计算了脾指数和胸腺指数。并对胸腺和脾脏两器官之间的关系进行了相关分析,对4种样品间的各测定项目作了比较。结果表明:4种样品中有2种合格2种不合格;黄芪多糖具有促进昆明鼠脾脏生长的作用,对昆明鼠胸腺的促进作用不明显,可能有一定抑制胸腺退化的作用;脾重和胸腺重之间、脾指数和胸腺指数之间均呈弱正相关关系;脾指数在反映黄芪多糖的生物活性方面是灵敏的,胸腺指数可以作为黄芪多糖生物活性检测的参考指标。
关键词:  黄芪多糖  生物活性检测  昆明鼠  脾脏重  胸腺重  脾指数  胸腺指数
DOI:
投稿时间:2018-12-17修订日期:2019-06-02
基金项目:
Analysis and Evaluation of Biological Activity Detection Method ofAstragalus Polysaccharide Injection
(Henan Province Animal Drug Feed Inspection Office)
Abstract:
In order to confirm the effect of the existing Astragalus polysaccharide bioactivity detection method, and to determine the role of thymus weight and thymus index in the detection of Astragalus polysaccharide bioactivity, the detection method provided by 《Chinese Veterinary Pharmacopoeia 2015 Edition (Part 2)》was used in the experiment. Biological activity of 4 Astragalus polysaccharides samples provided by the factoies in Henan were tested and the body weight, spleen weight and thymus weight of Kunming rats were measured, the spleen index and thymus index were calculated. Correlation analysis was performed on the relationship between the thymus and spleen organs, and the comparison between the four samples was made. The results showed that 2 samples were qualified and 2 were unqualified; Astragalus polysaccharide could promote the growth of spleen in Kunming mice,but it has no obvious effect on the thymus of Kunming rats and may has effect on inhibiting thymus degeneration; between spleen weight and thymus weight, the spleen index and thymus index were weakly positively correlated; the spleen index was sensitive in detecting the biological activity of Astragalus polysaccharides, and the thymus index could be used as a reference for the detection of Astragalus polysaccharide bioactivity.
Key words:  Astragalus polysaccharides  Astragalus polysaccharide bioactivity detection  Kunming mice  Spleen weight  Thymus weight  Spleen index  Thymus index.

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