葛根芩连汤对感染大肠杆菌小鼠血常规、血液内毒素及炎性细胞因子含量的影响

Effect of Gegenqinlian Soup on Blood Routine, Blood Endotoxin and Inflammatory Cytokines in E.coli-infected Mice

关键词:  大肠杆菌K₈₈  葛根芩连汤  死亡率  内毒素  炎性因子

Key words:  Enterotoxigenic Escherichia coli K88  Gegenqinlian Soup  mortality rate  endotoxin  inflammatory factor

摘要:

本文旨在研究葛根芩连汤预防及治疗感染大肠杆菌K88小鼠的血常规、血液内毒素及炎性细胞因子含量的影响。统计小鼠的死亡率，血常规检测小鼠血液中白细胞、红细胞及血小板的变化，以及采用显色基质鲎试剂法和ELISA方法检测感染小鼠血液里内毒素及炎性因子TNF-α、IL-1β、IL-6的含量变化。结果显示，预防试验中葛根芩连汤组小鼠死亡率为20％，治疗试验中葛根芩连汤组死亡率为30％，均极显著地低于模型组（P＜0.01），且与氟苯尼考组相比没有差异；葛根芩连汤组小鼠血液中白细胞指标均极显著地低于模型组（P＜0.01），红细胞及血小板指标均极显著地高于模型组（P＜0.01）；葛根芩连汤组小鼠白细胞、中性粒细胞及血小板数目极显著的高于氟苯尼考组（P＜0.01），葛根芩连汤组小鼠内毒素及TNF-α、IL-1β、IL-6的含量均极显著地低于模型组（P＜0.01）。结果表明：葛根芩连汤对感染大肠杆菌K88小鼠具有预防与治疗作用，而且在抑制早期炎性因子的释放，降低机体炎症反应水平方面优于氟苯尼考。

Abstract:

The purpose of this study was to investigate the effects of Gegen Qinlian Decoction on the prevention and treatment of blood routine, blood endotoxin and inflammatory cytokines in mice infected with E. coli K88. The mortality of mice was counted. The changes of white blood cells, red blood cells and platelets in the blood of mice were routinely detected by blood. The endotoxin and inflammatory factors TNF-α and IL in the blood of infected mice were detected by chromogenic matrix sputum reagent and ELISA. -1β, IL-6 content changes. The results showed that the mortality rate of the Kudzu Qilian Decoction group was 20% in the prevention test, and the mortality of the Gegen Qinlian Decoction group was 30% in the treatment trial, which was significantly lower than that of the model group (P<0.01), and with fluorine. There was no difference in the benidazole group; the blood leukocyte index in the mice of Gegenqilian decoction group was significantly lower than that in the model group (P<0.01), and the red blood cell and platelet indexes were significantly higher than the model group (P<0.01). The number of white blood cells, neutrophils and platelets in the Gegen Qilian Decoction group was significantly higher than that in the florfenicol group (P<0.01). The endotoxin and TNF-α, IL- in the Gegen Qinlian Decoction group. The contents of 1β and IL-6 were significantly lower than those in the model group (P<0.01). The results showed that: Gegen Qilian Decoction has preventive and therapeutic effects on E. coli K88 mice, and it is superior to florfenicol in inhibiting the release of early inflammatory factors and reducing the level of inflammatory response.