中兽药中砷含量检测方法研究

Study on the Method of Detection of Arsenic inTraditional Chinese Veterinary Medicine

关键词:  中兽药  砷  原子荧光仪  ICP-MS

Key words:  ：Traditional Chinese Veterinary Medicine  Arsenic  AFS  ICP-MS

摘要:

建立了检测中兽药中砷元素的两种检测方法,即原子荧光法(AFS)和电感耦合等离子体质谱法(ICP-MS),并对两种方法进行比较。固态或液态中兽药制剂经微波消解,赶酸、定容,采用原子荧光法及ICP-MS法测定砷的浓度。结果表明：原子荧光法及ICP-MS测定条件下,砷在0~50 μg.L^-1范围内的线性范围较好,相关系数R≥0.9990。在0.2~20.0 mg.kg^-1范围的添加回收率在80%~110%之间。批内、批间相对标准偏差均小于20％。两种方法操作简便,快速,重现性好,各有优势,适用于各种固态或液态中兽药制剂中砷的测定。

Abstract:

Two methods for detecting arsenic in veterinary drugs were established, namely atomic fluorescence spectrometer and inductively coupled plasma mass spectrometry (ICP-MS), the two methods were compared initiatively. The traditional Chinese veterinary medicinal preparations in solid or liquid forms were digested by microwave, acid driven and set to constant volume. Arsenic was measured by atomic fluorescence method and ICP-MS method. The results showed that under the conditions of atomic fluorescence spectrometry and ICP-MS, the linear range of arsenic was better in the range of 0~50 μg.L^-1, and the correlation coefficient was R≥0.9990. The recovery rate was between 80% and 110% in the range of 0.2~20.0 mg.kg^-1. The relative standard deviations within and between batches were less than 20%. The two methods are simple, rapid, reproducible, and each has its own advantages, both are suitable for the determination of arsenic in various solid or liquid veterinary preparations.